EREMASCACEAE IMPERFECTAE 279 



After 3 days at 37° C. in potato decoction, blastospores, spherical up to 

 7/i in diameter, each containing a big vacuole, sprouting at several points at 

 once with these daughter cells sprouting in turn without separating from the 

 mother cell. Hyphae formed of cells that lengthen with age, beginning about 

 20 X 2.5/A and ultimately attaining 30 x 1.5 fx. The protoplasm of the cells is 

 reduced to a thin layer along the wall. Verticils of spherical cells at the 

 septa; lateral branches rare. After 30 days intercalary and terminal chlamy- 

 dospores, which have a double membrane and a large vacuole, separate and 

 float free. Optimum temperature for growth 37° C. 



On Sabouraud agar at 37° C, colony round, dull white, with a smooth 

 margin at first, developing outgrowths composed of large, contorted hyphae, 

 which also grow down into the medium. On gelatin stab, culture white, with 

 denticulate margin, horizontal hyphae growing in depth of culture give a 

 growth of inverted cone shape. On potato decoction, white flaky sediment. 

 Ferments glucose and fructose, not maltose, galactose, sucrose, lactose, man- 

 nite, dextrin, or inulin. Neither acid formation nor clotting in milk. No 

 liquefaction of gelatin. 



Syring-ospora psilosis (Ashford) Dodge, n. comb. 



Monilia sp. Ashford, Jour. Amer. Med. Assn. 64: 810, 811, 1915; Amer. 

 Jour. Med. Sci. 150: 680-692, 1915. 



Monilia psilosis Ashford, Amer. Jour. Med. Sci. 154: 157-176, 1917 



[August] . 



Parasaccharomyces Ashfordi Anderson, Jour. Infect. Dis. 21: 341-387, Pis. 

 3-8, 1917 [October]. 



MyceloMastcmon psilose Ota, Jap. Jour. Derm. Urol. 28: [4], 1928. 



Mycotorula psilosis Langeron & Talice, Ann. Parasitol. Hum. Comp. 10: 

 47, 1932. 



Candida psilosis Almeida, Amiaes Fac. Med. Sao Paulo 9: 11, 1933. 



Isolated from a patient suffering from sprue in Puerto Rico. Pathogenic 

 to guinea pigs and rabbits. 



In young cultures cells are spherical or slightly ovoid ; in old cultures cells 

 more variable, ovoid, elongate, ellipsoid, spherical, or irregular. Giant cells 

 common, spherical, 3.5-5/a in diameter, Avitli refractile oil droplet. Septate 

 hyphae appear in hanging drop of gelatin and in old cultures. Hyphal cells 

 2-4/x in diameter, branching not uncommon but not t^'pical. Sprouting may 

 occur anywhere in young cultures but near ends of cells in old cultures. It 

 is the normal method of multiplication. 



On glucose agar streak, colony filiform, elevated, glistening, chalk-white, 

 and smooth. Later center may become rugose or pitted, margin even or fila- 

 mentous. On gelatin stab, growth at first filiform, later developing scattered 

 bushy clusters of filaments. In liquid sugar media and malt extract there is 

 a ring, but no pellicle. Liquid sugar media become at first acid, then more 

 alkaline. Glucose, maltose, and fructose fermented. "Occasionally sucrose 



