SACCHAROMYCETACEAE 321 



on gelatin grow more slowly and less abundantly than on agar, are grayish 

 white and of a mucous consistency. Almost no growth on coagulated serum. 

 After 4 days at 37° C, in bouillon, there is a sediment, but the liquid is clear. 

 No fermentation with sugars. Gelatin slowly liquefied. 



Saccharomyces Lemmonieri Sartory & Lasseur, C. R. Soc, Biol. 78: 48-49, 

 1915; Bull. Sci. Pharmacol. 23: 151-157, 1916. 



Isolated from a case of bronchitis and pulmonary congestion. Pathogenic 

 to guinea pigs, white mice, and rabbits. 



Cells 3.1-3.5/A in diameter, in age slightly elongate and clinging together 

 in short chains. In deposits only spherical cells found. On plaster block and 

 filter paper soaked in lactose solution, asci formed. These are 4-spored, the 

 spores being 2.5-3/^ in diameter. 



On Sabouraud agar, colonies punctiform, nonconfluent, white, dull. Cre- 

 nate margins 2-3 mm. high. At thirtieth day, colonies become chocolate brown. 

 On potato, colonies white, punctiform, confluent, center elevated and acumi- 

 nate, margins smooth. In age, colony cream white, becoming chalky on desic- 

 cation. On carrot, thick creamy colonies cover whole surface in a week. On 

 gelatin, growth is slow. On egg albumen, smooth punctiform colonies with 

 smooth borders appear on the ninth day. In broth with peptone, glycerol, 

 and glucose, growth rapid, pellicle quite thick, ring rather fragile, liquid tur- 

 bid, clearing with a sediment 2 mm. thick in 3 weeks. Sucrose inverted, glu- 

 cose, fructose, sucrose, and maltose fermented ; no action on lactose, galactose, 

 inulin, starch, or mannite. Milk coagulated on tenth day, curd digested by 

 the eighteenth. Liquefaction of gelatin begins on the eighth day. 



Saccharomyces Zimmeri Dodge, n. sp. 



Saccharomyces sp. Sartory, Sartory & Meyer in Zimmer, "Weil, Sartory, 

 Sartory & Meyer, Arch. Derm. Syphilis 167: 211-221, 7 figs., 1932. 



Isolated from abscesses from the gluteal region and upper thigh of woman 

 in Alsace. Pathogenic for guinea pigs. 



Cells ellipsoid, 4-5 x 2-3/i, forming chains up to 30/a long. Ascospores in 

 pus and on gypsum block, cultures smooth, 1.25/* in diameter. Asci formed 

 without copulation, and ascospores germinate directly. 



On potato agar and Sabouraud glucose agar, colonies white, homogeneous 

 with smooth margin, center becoming verrucose with yellowish Brockelchen 

 and radial furrows, finally grayish. On gelatin, colony spreading. On Meyer's 

 liquid medium, gray flocculent sediment and a pellicle after 3 days at 37° C. ; 

 this later sinks and a new one forms. Optimum temperature 27°-35° C, 

 optimum pH 5.8. Glucose, maltose, and sucrose strongly fermented and acidi- 

 fied, no action on fructose and lactose. Coagulated albumin unchanged. Milk 

 coagulated but not digested in 8 days. Gelatin not liquefied. 



Saccharomyces Sternoni Sartory, Sartory, Sternon & Meyer, BuU. Acad. 

 Med. Paris 107: 120, 121, 1932. 



Isolated from lesions in interdigital mycosis. Lesions reproduced on skin 

 of guinea pig. 



