EREMASCACEAE IMPERFECTAE 251 



occasionally separated by spherical cells. By the thirtieth day these long- 

 celled filaments are much like those of Casiellania Muhira. No chlamydospores. 



On Sabouraud agar, at 37° C., culture dull, white, circular. Later the 

 center yellows with radial striations, margin lobed with hyphal tufts pene- 

 trating the medium. On gelatin stab, appearance of inverted fir tree. In 

 potato decoction, white flaky sediment, no pellicle. Fermentation with glu- 

 cose and fructose, no fermentation of maltose, galactose, sucrose, lactose, man- 

 nite, dextrin, or inulin. Litmus milk slightly acid. Gelatin not liquefied. 



Castellania epidermica (Ciferri & Alfonseca) Dodge, n. comb. 



Blastodendrion intesiinale var. epidermicum Ciferri & Alfonseca, Centralbl. 

 Bakt. II, 83: 273-276, 1 pi., 1931. 



Isolated from a cutaneous mycosis of the face. 



Cells spherical in the center of the colony and on unfavorable media, 5fx 

 in diameter, occasionally 6 x 4/t, giant cells 7-8/x. Somewhat branched pseudo- 

 mycelium at the margins of the colony, cells elongate in short chains with thick 

 cell walls (1-1. 5/i). On liquid media up to 20 cells in chains. No ascospores. 



Optimum temperature 36°-38° C. On Sabouraud agar (pH 6.2), colony 

 white then yellowish, dense, creamy, thick, opaque with porcelain luster in 

 refracted light, later almost cloudy, center level and uniform, margins thinner 

 and grossly lobate, sometimes plumelike ; obscurely zonate in old cultures. On 

 grape must gelatin (pH 5.6), similar but more zonate. On Difco malt agar 

 (pH 6.8), similar, but growth more abundant and irregular. On Difco prune 

 agar (pH 6.8), growth poor, transparent, zones not evident, margins irregular 

 but nearly continuous. On potato and carrot, colony irregular, indefinite, 

 spreading, growth rapid. On corn meal agar (pH 7.2), surface very brilliant. 

 On Gorodkova agar (pH 6.8), growth poor, colonies small, almost transparent, 

 later whitish and opaque. On peptone glucose broth (pH 6.4), growth poor, 

 no pellicle, slight ring, sediment hyaline, agglomerated, grayish white, viscous, 

 liquid darkens and thickens. On autolyzed yeast (pH 6.4) and malt solution 

 (pH 6.0), growth very poor, no ring or pellicle, little sediment. Giant colony 

 of Will's type I, no crater, periphery thin, transparent, slightly denticulate, 

 odor of fermentation. No inversion of sugars, ferments glucose, fructose, 

 trehalose, assimilates glucose, fructose, maltose, and sucrose, no assimilation 

 of organic acids. Gelatin not liquefied. 



Castellania Mirandei (Velu) Dodge, n. comb. 



Cryptococcus Mirandei Velu, Bull. Soc. Path. Exot. 17: 545-547, 1924. 



Torulopsis Mirandei Almeida, Annaes Fac. Med. Sao Paulo 9: 10, 1933. 



Cryptococcus lacrymeatus Jeaume, Blastomycose des voies lacrymales de 

 I'ane au Maroc. These Doc. Vet. Avignon, 1926. 



Found in tear ducts only. Differs from Zymonema farciminosum in spec- 

 ificity for ass. Causes a pseudoinflammatory tumor characterized by a lym- 

 phocyte and plasmatic infiltration without gigantocellular reaction, vasculari- 

 zation, or sclerosis. 



