322 MEDICAL MYCOLOGY 



Cells spherical, 2-2.5/x in diameter, thin-walled, budding. Asci spherical 

 to ovoid, 3.75-4/t in diameter, each containing 4 ascospores, 1.25-1.5/i. in di- 

 ameter. No copulation observed. In the scales, the intracellular parasite 

 shows thin-walled cells. 



On beef agar, potato agar, and Eaulin agar, growth is good but less abun- 

 dant, colony dirty white. Growth poor on senim and egg albumen. On potato 

 juice, growth is good, pellicle shining white, thick. On Raulin acid medium, 

 a pellicle forms after 12 hours and falls to the bottom the next day. Growth 

 not luxuriant. A pellicle forms in milk on second day. Invertase secreted, 

 maltose fermented with evolution of gas. No action on lactose and levulose. 

 Milk not coagulated. Neither gelatin nor coagulated serum nor egg albumen 

 liquefied. 



Saccharomyces labialis Ribeyro, Anal. Fac. Med. Lima. 2: 122-127, 2 ph., 

 1918. 



Isolated from lesions on the lips of a young woman. Lesion on lower 

 lip worst, involving region adjacent to mucous membrane. Abscess in guinea 

 pig reproduced by inoculation. 



After growth for 24 hours at 37° C, in ordinary or glucose agar, cells 

 were predominantly 4-6/* in diameter with some smaller ones, 3-4/i. After 

 12-15 days, ovoid asci, 7-8/a with 4 ascospores to each. Ascospores fairly thin- 

 walled. No mycelial forms seen. 



On agar, growth abundant, colony dull white. Growth on potato good, 

 colony elevated, opaque, white. On beet, growth good, colony dark and oily. 

 Growth also good on carrot, colony oily and white. On SAveet potato, growth 

 poor, colonies white, slowly yellowmg. On gelatin, growth is mediocre, colo- 

 nies white, elevated, folded. Colonies on beef serum white and of the appear- 

 ance of porcelain. These do not darken with age. On hay infusion agar, 

 growth is scarce, colonies yellow white. In broth an abundant sediment forms. 

 In broth, to which maltose has been added, growth is good, the liquid is turbid, 

 and there is some sediment. In broth and glucose, levulose, sucrose, or man- 

 nite, growth is abundant, pellicle and sediment formed. With glucose and 

 levulose, acid is formed, with the other sugars slight. Medium is turbid. In 

 broth plus dextrin, inulin, or lactose there is less growth, medium remains 

 clear and sediment settles out, no acidity. In milk, growth is fair, sediment 

 forms. In a 1% aqueous solution of nutrose, growth is fair, liquid clear with 

 some sediment. Glucose and sucrose fermented, not the others. Milk not 

 coagulated, gelatin not liquefied. 



Saccharomyces annulatus Negroni, Ann. Parasitol. Hum. Comp. 7: 303- 

 308, 1929. 



Isolated from an abscess of the epididymis in Roumania. Pathogenicity 

 not known. 



Cells ovoid or ellipsoid, 3 x 4-6.5 x 11.5/x, on carrot (Fig. 69, 1). On both 

 carrot and Gorodkova agar, cells develop asci without copulation. Asci 1-4- 



