SACCIIAROMYCETACEAE 



323 



spored, most frequently 2. Spores spherical, smooth, tlattened at the poles, 

 about 3.5/x in diameter with a slight equatorial ring suggestive of Williopsis 

 Saturnus (Fig. 69, 3). Ascospores copulate in pairs within the ascus (Fig. 69, 

 3-7). The nuclei usually fuse and sprouting begins from the copulation canal. 

 Parthenogenesis is occasionally observed. The sexuality is at about the same 

 stage as in Sacchaiomycodes Luflwigii. Optimum temperature for budding 

 about 40° C, optimum for sporulation 28°, although both sprouting and 

 sporulation occur at room temperature (14-17° C). 



On malt agar, colony is dirty white, creamy in consistency, shining, moist. 

 Giant colony attains diameter of 4 cm. in 1 month, grayish with some small, 

 crateriform elevations in center. Malt extract becomes cloudy with a deposit 

 after a few days and a ring at the surface. Glucose, maltose, lactose, galac- 



Fig-. 69. — Saccharomyces annulatus. 1, sprout ceU ; 2, ascospores ; S-t, stages in copulation. 



(After Negroni 1929.) 



tose, and raffinose fermented, sucrose and xylose slightly, without inversion 

 in the case of sucrose. Fermentation of lactose denied by Dekker (1931). 

 Gelatin not liquefied. 



Dekker (1931) denied the existence of the equatorial ring on the asco- 

 spore, although she admitted she had observed one after staining with iron 

 hematoxylin. This she claims to be an artefact and reduces this species to 

 synonymy with the common beer yeast <S'. cerevisiae, although she does not 

 report this "artefact" on spores of the latter organism. 



Saccharomyces Jadini Sartory, Sartorj'-, Weill & Meyer, C. R. Acad. Sci. 

 194: 1688-1690, 1932. 



Isolated from abscesses on buttocks. Pathogenic to guinea pig. 



Cells 4-5/i, in diameter, asci 3.75 x 5/x; ascospores 2 or 4, about 1.25/t in 

 diameter. On solid media, some hyphae produced, 1.25-2.5 up to 25-30/u, long. 

 Asci also produced from these forms on gypsum blocks. 



