324 MEDICAL MYCOLOGY 



Oil solid media, white colony, with smooth margin and rough elevated 

 center, color darkening on aging. On liquid media, pellicle evanescent. Glu- 

 cose and maltose fermented, no action on lactose and fructose. Milk coagu- 

 lated and not digested, egg albumen and gelatin not liquefied. 



Doubtful Species 



The following species referred to Saccharomyces and rather imperfectly 

 described, seem anomalous in this genus and may belong near Bargellinia or 

 Deiaryomyces. 



Saccharomyces pseudotubercolaris Santori, Riforma Med. 19: 281-283, 

 1903. 



Saccharomyces infiltrans Casagrandi, Ann. Ig. Sperim. N. S. 9: 141-155, 

 1899; Riforma Med. 19: 281-283, 1903 (nomen nudum). 



Isolated from pseudotubercular lesions resembling sarcoma in the abdomi- 

 nal cavity of fowl. Pathogenic to laboratory animals. 



Yeast cells spherical to ovoid, 1.5-2/1,. On old cultures on solid media and 

 in some liquid media, elongate ellipsoid cells occur in chains of more than 3 

 cells. Asci thick-walled, 2-4-spored. 



On acid glucose agar, colony humid, white, thick, opaque, surface rough, 

 margins sinuous lobed. On alkaline agar, colony shining, white, thinner. On 

 gelatin, colonies whitish, moist, circular with irregularly lobed margins. On 

 potato, colonies whitish, thick, at first moist becoming dry, surface first smooth 

 becoming rough with sinuous lobes. On sugar broths, sediment formed but no 

 ring or pellicle. No production of pigment, no hydrolysis of starch, no inver- 

 sion of sucrose or amygdalin. No fermentation of sugars, milk not coagu- 

 lated, gelatin not liquefied. 



Perhaps the following unnamed organism should be referred here. 



Saccharomyces sp. Darmagnac & Barletti, Bull. Soc. Centr. Med. Vet. 68: 

 157-163, 5 figs., 1914. 



Isolated during an epizootic of turkeys, causing rapid death with primary' 

 lesions on the lungs and liver, occasionally also on the peritoneum, intestines, 

 kidneys, and the myocardium. Yeast cells found in pseudotubercular lesions. 



Yeast cells 6-lOfx in diameter, while in old cultures cells elongate, tending 

 to form a pseudomycelium. After two months, certain cells enlarge and con- 

 tain ascospores(?) (figured as 2 per ascus). On plaster blocks these forms 

 appear in a few days. The filaments are of irregular diameter and apparently 

 form chlamydospores. 



Colonies on glucose glycerol agar, -whitish, creamy, not very adherent. 

 On Sabouraud agar, white circular colonies, surface slightly convex. Colony 

 on potato white, shining, creamy, becoming broAvnish in age. Flat, grayish 

 colony on carrot. On gelatin, good surface growth. In broth, sediment on sides 

 and bottom of tube, liquid remaining clear. Under anaerobic conditions, 

 growth very slow and colony not characteristic. Gelatin not liquefied. 



