SACCHAROMYCETACEAE IMPERFECTAE 331 



ovoid, 2-22/i,, with irregular and distorted forms. Large vacuoles present, 

 capsules more conspicuous. Cell division in all planes; no ascospores. 



On malt agar, colon^^ whitish, translucent, elevated, becoming opaque, 

 yellowish white, margins beveled, finally yellowish brown and up to 6 mm. 

 thick. On malt gelatin, colonies confluent, flat margins elevated, centers 

 slightly depressed, surface dull, smooth, waxy, yellowish white. On blood 

 serum, colonies milk white, elevated, slightly slimy, glistening becoming yel- 

 lowish, drying chalky, resembling daubs of white paint. On potato, colony 

 elevated, margins regular, verrucose, white or slightly yellowish becoming 

 gray or drab. On malt extract, liquid clear, sediment abundant, granular to 

 flocculent, ring well developed, pellicle very finely granular or cheesy, be- 

 coming thick. On glucose broth, little or no pellicle, sediment grayish white. 

 No action on milk. (lelatin not liquefied (Sanfelice) or slowly liquefied after 

 a week, becoming complete in 5-6 weeks (Weis), 



Cryptococcus Plimmeri (Costantin) Neveu-Lemaire, Parasitol. Anim. 

 Domest. 60, 1912. 



Saccharomyces sp. Plimmer, Centralbl. Bakt. 25: 805-809, 1899. 



Saccharomyces Plimmeri Costantin, Bull. Soc. Myc. France 17: 147, 1901. 



Torula Plimmer Weis, Jour. Med. Res. 7: 280-311, Pis. 21-24, 1902. 



Torulopsis Plimmeri Almeida, Annaes Fac. Med. Sao Paulo 9: 10, 1933. 



Found in about half of a series of cancer cases examined by Plimmer. 

 Pathogenic to guinea pig in both intraperitoneal and subcrural injections. 



In pellicle of young cultures, cells 4-8/i,, without capsules, vacuolate with 

 many oil globules. In sediment, cells larger, 2-10/a, capsules on very large 

 cells, not on small ones. In old cultures, cells 2-16/* with 1-3 large vacuoles, 

 capsules highly developed; cells in chains of 3 cells, the middle cell usually 

 smaller; cell division in one plane only; no ascospores. 



On malt agar, colony whitish, translucent, elevated, becoming radially 

 furrowed and zonate, center pure yellow Math white margin. On malt gelatin, 

 colonies confluent, smooth, center not depressed, margins not elevated, white, 

 then yellowish. On blood serum, growth slight, whitish, flat, giving the ap- 

 pearance of daubs of paint. On potato, colony yellowish brown, surface con- 

 voluted, margin regular. In malt extract broth, liquid clear, pellicle delicate 

 not settling to the bottom, sediment cheesy to coarsely granular, highly de- 

 veloped, ring heavy, opaque. In glucose broth, pellicle well developed at 25°, 

 little or none at 37° C; sediment very slight. Growth good in litmus milk 

 but no change in medium. No fermentation of sugars. Gelatin not liquefied 

 (Plimmer), very slowly liquefied after one week, becoming complete in 5-6 

 weeks (Weis). 



Cryptococcus lithogenes (Sanfelice) Vuillemin, Rev. Gen. Sci. Pures Appl. 

 12: 739-741, 1901. 



Saccharomyces litogenes Sanfelice, Centralbl. Bakt. I, 18: 521-526, 1895. 



Toriila Sanfelice Weis, Jour. Med. Res. 7: 280-311, Pis. 21-24, 1902. 



Blastomyces lithogenes Sasakawa, Centralbl. Bakt. I, 88: 274, 1922. 



