SACCHAKOMYCETACEAE IMPERPECTAE 333 



and inulin. Sucrose inverted. Milk becomes alkaline without other change. 

 Malt gelatin slightly liquefied in 57 days, ordinary gelatin in 26 days. 



Cryptococcus meningitidis Dodge, n. sp. 



Torula histolytica Harrison, Trans. R. Soc. Canada, Biol. Sci. 22: 187-225, 

 5 ph., 1928. 



Isolated from a case of cystic blastomycosis of the cerebral gray matter. 



Cells spherical, even in size, averaging 3.5/4 in diameter; on 56-day-old 

 cultures 3-6/x in diameter, averaging 4.2ju,; oil globules present, the older cells 

 having a mucinous capsule. 



On malt extract agar, growth is Avhite and shining, slightly spreading, in 

 age becoming yellow then brown, the growth more massive. In malt gelatin, 

 colony white, waxy, elevated, with radiate markings, later forming a funnel- 

 shaped depression. Giant colony is whitish, shining, slightly rugose, margin 

 lobate, center slightly depressed. On potato, colony elevated, white, shining, 

 and spreading. In malt extract, a ring and pellicle develop with turbidity 

 and heavy sediment. Ring in solutions of maltose, lactose, sucrose, raffinose, 

 salicin, and dextrin, none in glycerol, sorbite, or inulin. Under olive oil, 

 capitate colonies form, growth slight. Grows well between 25° and 37° C. 

 Sucrose not inverted. In acid sugar media (pH 5.2), pellicle, turbidity and 

 sediment in glucose, mannose, galactose, fructose, and xylose. In milk, ring 

 forms, reaction slightly alkaline, no coagulation. Gelatin liquefied in 26 days. 



Lynch & Rose (1926) report slight liquefaction of gelatin after 3 months, 

 also slight acidity in maltose (and after 3 months in sucrose). Rappaport & 

 Kaplan (1926) report their organism to be gram -positive when young. Growth 

 best on carbohydrate media and under low oxygen tension. 



Cryptococcus rotundatus (Redaelli) Dodge, n. comb. 



Torulopsis rotundata Redaelli, I Miceti come Associazione Microbica nella 

 Tubercolosi Polmonare Cavitaria 46, 1925 (fide Lodder, Anakosporogenen 

 Hefen 1: 170-172, 1934). 



Isolated from a case simulating tuberculosis of the lung. 



Cells spherical 5-6.5/a in diameter, giant cells to 10/t. On malt agar, cells 

 slightly ovoid. 



On carrot agar, colonies white to slightly pinkish, surface dull. On malt 

 agar, colonies yellowish, slightly reddish, humid, smooth, slimy, margin smooth. 

 On malt gelatin, colonies yellowish, dull, center elevated, margin somewhat 

 sinuous and slightly shining. On malt extract, a ring and sediment, the whole 

 fluid becoming slimy. 



Cryptococcus Gotoi Dodge, n. sp. 



Hefe Goto, Mitt. Med. Fak. Univ. Tokyo 15: 75-101, Pis. 9-10, 1915. 



Isolated from a case of blastomycotic meningitis in Japan. Fatal for 

 mice, rats, guinea pigs, and rabbits, but not for dogs. 



Cells 3-6/x, walls thin when young, becoming very thick-walled when old. 

 No mycelium or ascospores. Capsule well developed. 



