336 MEDICAL MYCOLOGY 



latter the immediate cause of death about 3 months after the appearance of 

 the first cutaneous lesion. Patient a native of Spain, resident twenty years 

 in the Argentine. Pathogenic to rabbits, guinea pigs, white rats, and mice, 

 with reproduction of lesions both microscopically and macroscopically. 



Examination of pus from abscesses showed spherical yeast cells, very 

 variable in size, 7-30/a, sprouting or not, possessing a thick, smooth double- 

 layered wall, quite visible and enclosing a somewhat granular substance. Some 

 cells have 2-4 granules, 1-2/^ in diameter. Some large cells with thick, gelatinous, 

 hyaline wall, 5/x or more thick. Cells solitary or in chains of 2, 3 or 4. Some- 

 times vacuolate with protoplasm along wall in ring or half moon and showing 

 a few black corpuscles. Occasionally spore wall ruptures, allowing cell con- 

 tents to escape. 



Yeast cells were also encountered in the tissues. Under culture the or- 

 ganism always has appeared with spherical cells, 3-7/* in diameter, single or 

 sprouting, with thick external membrane and somewhat granular content. 

 Hyphae never seen. Gelatinous sheath never seen in culture. While in the 

 pus, the organism was hard to stain, being gram-negative and only faintly 

 tinted with Leishman and May-Griinwald-Giemsa stains ; the cultivated organ- 

 isms stain readily. 



Optimum temperature 25°, little growth at 37° C. 



On Sabouraud agar, abundant growth in 24 hours, colonies cream white, 

 hemispheric, not confluent, humid above, not adhering to medium below. 

 When growth is very abundant, culture appears dry and oily. Growth in 

 plain agar similar, but less abundant. On poor nutritive agar, abundant 

 growth in 24 hours. On potato, with 8% glycerol, good growth in 48 hours, 

 colonies confluent, moist, grayish white, liquid without turbidity, with sedi- 

 ment at bottom of tube. On carrot with 8% glycerol, regular growth after 

 48 hours, colonies isolated, white, hemispheric, moist, sediment at bottom of 

 tube with liquid clear. On coagulated human serum, growth scarce after 24 

 hours and poor thereafter, no color production or liquefaction. When inocu- 

 lated by stab, growth scant at first. After 20 days infundibuliform growth 

 and liquefaction at bottom. Scant growth also on coagulated albumen, with 

 neither liquefaction nor pigment formation. On Gougerot gelatin (stab), sur- 

 face growth at 48 hours, colonies flat, white with scalloped edge and moist 

 surface. On Drigalski-Conradi medium, no growth in 76 hours. In plain 

 broth or peptone solution or acid Raulin solution, after 48 hours poor, pow- 

 dery growth along the walls of the tube and forming a scant sediment at the 

 bottom, no turbidity and no pellicle. In Sabouraud broth, after 3 days, 

 medium becomes turbid with abundant sediment and white pellicle which 

 falls to the bottom after being shaken. Does not ferment any of the usual 

 sugars, does not liquefy gelatin or coagulate milk. 



It seems probable that the following organism should be referred here. 



Torula sp. McGehee & Michelson, Surgery, Gyn. Obstet. 42: 803-808, 6 figs., 

 1926. 



