ENDOMYCETALES 143 



Reported occasionally from sputum but in none of the cases has patho- 

 genicity been clearly shown. Beauverie & Lesieur (1912) reported a case of 

 phthisis with the organism in the mucopurulent sputum. Grigorakis and Peju 

 (1922) also report a case. Shrewsbury (1930), in a monograph on the genus, 

 reports his strain 209 isolated from sputum from a case of chronic bronchitis 

 along with Monilia, and an unidentified yeast. Shrewsbury was unable to 

 find any pathogenicity for his strain on experimental animals. 



Pseudomycelium formed on many media, especially in pellicles on liquid 

 media. On carrot at 26° C. in 6 days, cells spherical or ovoid, 3-8 x 2.5-6/*. 

 Sporulation on carrot juice in the pellicle after 55 hours, cells 3-8/a in diameter. 

 Asci spherical, ascospores typically cucullate. 



Colonies cream white, smooth, and shining. Usually developing a strong 

 odor of fruit ethers. 



Hansenula bispora (Mattlet) Nannizzi, Tratt. Micopatol. Umana [Pol- 

 lacci] 4: 134, 1934. 



Saccharomyces (Willia) Mspora Mattlet, Ann. Soc. Beige Med. Trop. 6: 

 32, 33, 1926. 



Isolated from stools of patients with various degrees of dysentery. Patho- 

 genicity not proved; animal experiments not yet reported in detail. 



In potato decoction at 37° C. after 3 days, spherical or ovoid cells con- 

 taining a vacuole and granulations, 2-10/*, budding, larger cells 6-7/* or rarely 

 pyriform 11 x 7/*, thick-walled granular with little or no vacuole, other cells 

 elongate, swollen at one end, the smaller tip of one against the swollen tip 

 of the other and showing isogamous copulation. After about 10 days, oil 

 droplets abundant in most of the cells or occasionally droplets unite into one 

 large drop, copulation forms rarer. Little change in appearance after 30 days. 

 On Gorodkova agar asci appear after 5 days, thick-walled, containing 2 

 cucullate ascospores 6 x 3/*. On Sabouraud agar colonies white dull, circular 

 with even margin, in age the center becomes yellow and a few radiating folds 

 develop. On gelatin stab, slight liquefaction with some gas formation. In 

 potato decoction, abundant deposit of yellowish white clots which dissolve in 

 the liquid on shaking. Optimum temperature 37° C. Litmus milk slightly 

 acid. Acid and gas on glucose, fructose, maltose, galactose and sucrose; very 

 slight acid on dextrin, no action on lactose and mannite. 



HANSENIOSPORA 



Hanseniospora Zikes, Centvalbl. II, 30: 145, 1911. 



Yeast cells citriform or elongate ovoid, vegetative multiplication by bipo- 

 lar sprouting; no pellicle on malt, spores spherical at first, then cucullate 

 (ability to form spores easily lost on cultivation), only glucose slightly fer- 

 mented, nitrate not assimilated, practically no growth on ethyl alcohol. 



Hanseniospora Guilliermondii Pijper, Proc. Sect. Sei. K. Akad. Wetensch. 

 Amsterdam 31: 989-992, 2 figs., 1928. 



Isolated from onychomycosis of European woman in Pretoria; patho- 

 genicity quite probable but not proved. 



