332 MEDICAL MYCOLOGY 



Torulopsis Uthogenes Almeida, Annaes Fac. Med. Sao Paulo 9: 10, 1933. 



Isolated from the lymphatic ganglia of an ox, which died of generalized 

 carcinoma. Pathogenic for guinea pig and mouse, but not for sheep. (Strain 

 studied by Weis was isolated from adenocarcinoma of human ovary by San- 

 felice.) 



In tissues cells spherical, variable in size, containing rounded or angular 

 masses of calcareous material. In pellicle, cells spherical, 1-8/x,, oblong and 

 misshapen cells observed during active sprouting ; capsules absent or ex- 

 tremely delicate. In sediment, cells 3-12/a with a capsule, protoplasm granular 

 with oil drops and vacuoles. In old cultures capsules 1-1. 5yu, thick, cells often 

 embedded in a mass of gel, many resting cells present ; cells dividing in all 

 planes, no ascospores present. 



On malt agar, colony at first whitish, elevated, becoming opaque, yellow, 

 center folded, margins even, sharply beveled. On malt gelatin, colonies con- 

 fluent, flat, somewhat elevated at the margin Avith slightly depressed centers, 

 surface dull, smooth, waxy, yellowish white. On blood serum, colonies white, 

 elevated, slimy, glistening, yellow, growth scant, drying chalky, suggesting 

 daubs of white paint. On potato, colony very dark brown, margin very ir- 

 regular, surface glistening and corrugated. In malt extract broth, liquid 

 clear, pellicle at first very delicate, later heavy and membranous; sediment 

 slight until after fall of first pellicle, then heavy, cheesy, and flaky; ring well 

 developed. On glucose broth, pellicle developed, white becoming yellowish, 

 little sediment grayish white. No fermentation, no action on milk, gelatin 

 not liquefied (Sanfelice) or slowly liquefied after one week, the action com- 

 plete in 5-6 weeks (Weis). 



Cryptococcus nasalis (Harrison) Dodge, n. comb. 



Torula nasalis Harrison, Trans. R. Soc. Canada, Biol. Sci. 22: 207, 1928. 



Torulopsis neoformans race nasalis Lodder Anaskosporogenen Hef en 1 : 176, 

 1934. 



Isolated from nasal tumor in horse by K. F. Meyer (1912). 



In young colonies, cells spherical, average diameter 4/a. In 57-day-old 

 cultures, cells still spherical, varying from 3.5 to 7/a in diameter (average 5/t), 

 oil globules present. Growth good between 25° and 37° C. 



On malt agar, growth is elevated, shining, spreading, and white, later 

 darker in color and more massive. On potato, colony white, raised, dull, 

 spreading, becoming darker in age. On malt gelatin, colony elevated, white, 

 waxy, radiately striate, forming a funnel-shaped depression in 57 days. Giant 

 colony is dull, white with slightly elevated and lobate margin and depressed 

 center. Slight growth under olive oil. In malt extract, a creeping pel- 

 licle appears, then a heavy ring with cloudiness and thick sediment. No 

 fermentation of sugars. Acid produced in glucose, fructose, mannose, man- 

 nite, xylose, maltose, sucrose, and dextrin; forming a pellicle and turbidity 

 which clears as the sediment forms in all sugars except rhamnose, glycerol, 



