SACCHAROMYCETACEAE IMPERFECTAE 347 



Isolated from pulmonary myxoma of a guinea pig. 



Cells small, sprouting, dark. 



Colonies on agar, circular, whitish, covering the whole surface. On 

 potato, colonies grayish and chocolate color. In glucose broth, sediment but 

 no pellicle. Good growth in milk. Alcoholic fermentation of malt extract. 

 Gelatin not liquefied. Little growth on gelatin, less on blood serum, and very 

 little on beef broth. 



Quite probably this species should be transferred to the Toruleae. 



Cryptococcus sp. Breed, Arch. Int. Med. 10: 108-121, Figs. 1-6, 1912. 



Isolated from the respiratory tract of man, also from one case of severe 

 vaginitis. Pathogenic for rabbits, white rats, guinea pigs, and monkeys. Cure 

 effected by medication with sodium iodide. 



Cells size of a red blood corpuscle. No spores found. Grows between 

 18° and 69° C, though slight at room temperature. Killed in one hour at 

 71° C. Viable dry for 2 years. 



In 12-24 hours at 37° on glucose and glycerol agars there is a profuse 

 creamy growth. On potato slant under similar conditions, a slight film forms. 

 Grows on plain or glucose broth with production of heavy sediment (and foam 

 with latter), on shaking. Good growth on plum and grape decoctions but 

 no foam. Growth on litmus milk creamy white with soft curd in 24 hours. 

 Glucose fermented. Growth but no fermentation on lactose, raffinose, inulin, 

 and mannite. 



Cryptococcus de Bumier (cas Th) Ota, Ann. Parasitol. Hum. Comp. 2: 

 44-45, Fig. 5, 1924. 



Isolated from a case of epidermomycosis. 



On malt agar, at 25° C. in day-old cultures, cells ovoid or slightly elon- 

 gate, solitary or in chains of a few cells, 6-8 x 4-5/i., wall thin. After a week, 

 cells 12 X 10/t, spherical or ellipsoid, wall thicker. 



On malt agar, colony grayish white becoming yellowish, smooth, humid, 

 margin definite. On malt extract, forming a thin ring and sediment. 



TORULOPSIS 



Torulopsis Berlese, Giorn. Viticoltura ed Enologia 54, 1894. 



I have been unable to locate the original publication of this name, but it was designed 

 to end the confusion caused by the application of Torula to two different groups of organ- 

 isms (see p. 327). This has been applied to the asporogenous yeasts, following the traditional 

 application of Torula by Turpin, Mem. Acad. Sci. 17: 1-88, 1838, Pasteur, fitudes sur la Biere 

 73, 1876, Hansen, Medd. Carlsberg Lab. 2: 87-93 [47-52], 1888, Will (1916), etc. Turpin prob- 

 ably included both asporogenous and sporogenous strains and emphasized fermentation; 

 Pasteur held the same concept, but denied fermentation. Hansen was the first to use the name 

 in the modern sense, applying it to asporogenous yeasts without regard to fermentative power. 



In proposing this genus name, Berlese gave a good resume of the characters given by 

 Hansen, limiting its application definitely to the forms without mycelium, and excluding the 

 species with citriform cells (see Pseudosaccharomyces) . Will (1916, 1917) still further re- 

 stricted the generic concept (although still using the name Torula), defining it as follows: 



