SACCHAROMYCETACEAE IMPERPECTAE 349 



and moister. On Raulin's liquid, colonies rose to clear vermilion, margin 

 irregular, surface shining to oily, becoming 1 mm. thick and spreading over 

 the whole surface of the medium. On 5% or 10% glucose solution no pellicle 

 appears, very slight pigment, medium turbid, no fermentation (?). Organism 

 attacks oil used to secure anaerobiosis, apparently assimilating glycerol and 

 leaving floating masses of stearic acid crystals at interface of oil and medium. 



Torulopsis bronchialis Ciferri & Redaelli, Atti 1st. Bot. R. Univ. Pavia, 

 ni, 2: 245-248, 283, 1925. 



Bhodotorula hroncliialis Lodder, Anaskosporogenen Hefen 1: 91-93, 1934. 



Isolated from sputum of a patient with bronchopneumonia, not pathogenic 

 for laboratory animals. 



Cells ovoid, at first hyaline, then guttulate, 7-7.5/a in diameter up to 

 9-9.5 X 10/x, often forming short chains. On malt extract, 3-4 x 5-8/t, singly 

 or in pairs. No spores or mycelium. 



On carrot agar, colonies dense, creamy, radially folded, opaque, shining, 

 oehraceous to reddish. On malt agar, colony intense orange, moist, smooth, 

 margin pellucid. On gelatin, colonies red orange, verrucose radially folded. 

 On carrot, colonies small, uniform, surface irregular, reddish. On potato, 

 colonies spreading, flesh red, uniform, margins smooth, becoming irregularly 

 granulose. On malt extract, a pulverulent pellicle formed by confluence of 

 single colonies with granular sediment. On glucose broth, similar, pellicle 

 pink. Methylene blue reduced slowly. On milk, pellicle almost white, casein 

 precipitated after one month and very sowly digested. No alcoholic fermenta- 

 tion ; acid with glucose, fructose, galactose, sucrose, lactose, and inulin. Lac- 

 tose not assimilated (Lodder). 



Torulopsis mena (Fontoynont & Boucher), Dodge, n. comb. 



Cryptococcus mena Fontoynont (nom. nud.), Bull. Mem. Soc. Chirurg, 

 Paris 48: 439-442, 1922; Fontoynont & Boucher, Ann. Derm. Syphiligr. VI, 

 4: 213-235, 3 figs., 1923; Ota, Ann. Parasitol. Hum. Comp. 2: 45-47, 1924. 



Isolated from ulcerations on great toe and legs, with severe lesions on 

 chest due to earlier unsuccessful surgical interference when lesion was mis- 

 taken for cold tuberculous abscess. Medication with potassium iodide, etc., 

 wholly unsuccessful ; in fact, KI made lesions worse. Final cure effected by 

 internal and external applications of methylene blue. Even in pure cultures, 

 0.0015-0.002% methylene blue checked growth. Organism secretes a toxin 

 fatal to rat and guinea pig. No agglutination of Cryptococcus with patient's 

 blood nor with other blood, but agglutinations prompt in presence of meth- 

 ylene blue. 



Yeast cells ellipsoid, 4-5ju. in diameter, sprouting on second to fourth day. 

 By thirtieth day cells become more rounded, cell walls smooth. Optimum 

 temperature 20° but growth good also at 37° C. 



On glucose agar, colony rose, finally becoming brick red, attaining di- 

 ameter of 2.5 cm. in 5 days. On maltose agar, colony rose brown. On poor 

 agar, colony develops very slowly with production of bad odor. On potato. 



