350 MEDICAL MYCOLOGY 



colony rose, terra cotta, then red brown. On glucose gelatin, colony rose by 

 reflected light, grayish white beneath and by transmitted light, later becom- 

 ing color of eosin powder. In glucose broth, liquid remains clear with sediment 

 which is white at first, slowly turning rose. Gelatin slowly liquefied. Pigment 

 production varies with temperature, being less at higher temperature, also 

 less when organism is freshly isolated. 



Var. Mekimdu (Mattlet) Dodge, n. comb. 



Cryptococcus Mekundu Mattlet, Ann. Soc. Beige Med. Trop. 6: 14, 15, 1926. 



Isolated from sputum in case of bronchitis in Belgian Congo. Patient 

 had been weakened by malaria, bronchitis accompanied by a low continuous 

 fever with periodic exacerbations suggesting undulant fever, but no aggluti- 

 nation with Micrococcus melitensis. Patient removed to Europe shortly after 

 organism was isolated and not further studied. 



In sputum, cells 3-4/* in diameter. On potato decoction at 25°, cells spheri- 

 cal or ovoid, walls thin homogeneous on third day, sprouting, 2-6/a in diameter. 

 Older cells have thick walls. 



On Sabouraud agar, colony red, circular, soon flowing to bottom of slant. 

 On potato decoction, rose sediment on bottom and walls, clots disappearing on 

 agitation. Gelatin liquefied, optimum temperature 25° C. Slight acid pro- 

 duction in, and coagulation of, milk. Acid with glucose, fructose, galactose, 

 sucrose, and lactose. 



Close to T. mena, but interior of cells less granular. 



Torulopsis Sang^orgii Dodge, n. sp. 



Cryptococcus sp. Sangiorgi, Centralbl. Bakt. I, 63: 58-62, 1912. 



Isolated from the peripheral blood stream and from the liver of a dog 

 after 5 months of fever. Pathogenic for dog, slightly for rabbit. 



Cells in liquid media ovoid or ellipsoid, on solid media similar or very 

 rarely spherical. Extreme dimensions 4-6 x 3-5/*. Sprouting from the ends 

 of cells on solid media producing chains of 2-4 cells but no true hyphae. 

 Growth at 20° and 27° C, the higher temperature best for acid media. 



On Sabouraud agar, colony rosy, moist, creamy. On lactose agar, colony 

 very superficial. Growth slight on plain agar, blood agar, and coagulated 

 horse serum. On potato, colony thick, spreading, creamy, moist below, and 

 powdery above. Colonies orange yellow on most media but grayish white on 

 blood agar. On gelatin, growth slow% penetrating the medium. In plain 

 broth, liquid remains clears, no pellicle, and slight sediment. In glycerol 

 broth, sediment on bottom and walls but no pellicle. No indol formation in 

 peptone solution. No fermentation of glucose, maltose, lactose, dextrin, or 

 inulin, no inversion of sucrose. Milk coagulated in 27-30 days. Gelatin not 

 liquefied in 90 days. 



Torulopsis mitis (Mazza, Stabile de Nucci & Canal Feijoo) Dodge, n. comb. 



Cryptococcus mitis Mazza, Stabile de Nucci & Canal Feijoo, Bol. Inst. 

 Clin. Quirurg. Univ. Buenos Aires 5: 293-308, 21 figs., 1930. 



I 



