SACCIIAROMYCETAC'EAE IMPERFECTAE 351 



Producing pustules, origiiiall}^ appearing on tiie face, subsequently spread- 

 ing to all external parts of the body, in a 10-year-old Argentine boy. Lesions 

 secreting a serous, sanguinolent liquid. ^Medication with increasing doses of 

 potassium iodide caused eventual complete cicatrization of the lesions. Emul- 

 sion of yeast isolated and reinjected into dermis of patient caused local reac- 

 tion. Emulsions of organism cultivated on ISabouraud agar injected intra- 

 peritoneally into Cebus monkey, rabbit, guinea pig, two mice and two rats, 

 nonpathogenic. Scarifications and rubbing with organism caused no lesions 

 on monkey. Subcutaneous injection into white rat caused appearance of 

 nodule v\diich, removed and examined, showed presence of organism. 



On Sabouraud agar, cells round, averaging 5/x. in diameter, possessing a 

 thick membrane with an unevenly granular content. No hyphae or asci seen 

 after long observation. Reproduction by sprouting. Gram-positive. 



On Sabouraud agar, at 37° C, no growth. At room temperature after 

 3 days, orange red colonies, slightly elevated at center, scalloped margin. 

 After 10 days colonies elevated, margin undulate, with a few radial furrows. 

 Organism grows well on potato or carrot and glycerol, also on plain agar and 

 Gougerot gelatin and always at room temperature. In plain broth, slight 

 cloudiness in 48 hours. In Sabouraud broth, general turbidity. Fermenta- 

 tion after 48 hours with raffinose and fructose, none with sucrose, inulin, 

 lactose, mannite, maltose, galactose, sorbite, glucose, dextrin, or arabinose. 

 No indol formation. Milk not coagulated. 



Torulopsis glutinis (Fresenius ampl. Harrison) Dodge, n. comb. 



Cryptococcus glutinis Fresenius, Beitr. Mykol. 2: 77-78, 1852. 



Saccharomyces glutinis Cohn, Beitr. Biol. Pflanzen 1: 187, 1875 (cf. Hansen, 

 Medd. Carlsberg Lab. 1: 253-264 [81-88] 1879, etc.). 



Torula glutinis Pringsheim & Bilewsky, Beitr. Biol. Pflanzen 10: 118, 1910. 



Rhodotorula glutinis Harrison, Trans. R. Soc. Canada 22: 187, 1928 [cf. 

 Lodder, Anaskosporogenen Hefen 1: 65-68, 1934]. 



The following pink yeasts rather imperfectly described are often referred 

 here but may belong to other species of this genus, 



Saccharomyces roseus Engel, Bull. Soc. Sci. Nancy 6: 1, 1877 (cf. Mag- 

 giora & Gradenigo, 1896). 



Saccharomyces rosaceus Crookshank, Introd. Pract. Bact. 224, 1886 [cf. 

 Vuillemin & Legrain, Arch, de Parasitol. 3: 260-266, 1900]. 



Saccharomyces Fresenii Schroeter, Kryptog. Fl. Schlesien 3: 2: 208, 1893. 



This species seems to be primarily a saprophyte, not pathogenic for labo- 

 ratory animals, although from time to time reported as isolated from otitis 

 media, etc., perhaps as a contaminant, as this group of pink yeasts is fre- 

 quently found in old laboratory cultures. The following description is based 

 on culture of Pringsheim & Bilewsky isolated from air, amplified by Har- 

 rison and Lodder who studied the same strain. 



