SACCHAROMYCETACEAE 311 



Isolated from abscess on neck, not paint'nl, in some cases healing spon- 

 taneously. No organism seen in the tissue, discharge showed a yeast and a 

 streptococcus. On guinea pig caused abscess which healed spontaneously. 



Growth slow on solid media. In liquid media showing some budding cells, 

 cells 3-4/A, filled with dense protoplasm and asci 4-5 (-12)/x in diameter with 

 4 spores, and at the bottom a zoogloea of round cells. Cells more elongate on 

 repeated subculture. With beginning of ascospore formation in the culture, 

 sprouting largely ceases. Copulation heterogamic, often between a sprout and 

 an adult cell. Parthenogenesis occasional. Ascospores spherical, slightly 

 rough, with one to several oil drops, 2-3/a in diameter. 



Colonies on sugar agar somcAvhat hilly, shining, viscid, with a brownish 

 mucous deposit in water of condensation. Ascospores appear after the twelfth 

 day, their number finally, after repeated transfer, being reduced to one. On 

 usual media, colonies dirty white. Giant colony on potato agar attains a 

 diameter of 5 cm. with elevated mounds at the center and a few radiating 

 striae, margin crenulate. On potato juice, colony is humid and shining, later 

 becoming ochraceous. Growth best on potato media, giving colonies of a cream 

 color which later darkens to chamois yellow. Optimum temperature 37° C, 

 growth slow at room temperature. In liquid media, there is a brownish yellow 

 sediment, no pellicle, but a slight ring. Sucrose inverted, sucrose, and glucose 

 fermented, no action on other sugars. No effect on gelatin or coagulated serum. 



Debaryomyces emphysematosus Ota, Derm. Woch. 78: 284-289, 312-316, 

 1924. 



Hefe emphysematosus Sasakawa, Centralbl. Bakt. I, 88: 273, 1922. 



Organism rtceived from Hautklinik, Bern, marked Sacc. emphysematosus. 

 It is nonpathogenic for laboratory animals. 



On malt extract agar after 24 hours appear cells, usually solitary, very 

 rarely in chains, 2-5/t in diameter, no fat globules. After 3 days on carrot, 

 asci appear. Both heterogamous and isogamous asci smaller than in other 

 species. Spores very slightly verrucose. 



Glucose, fructose, sucrose, and raffinose fermented. 



Debaryomyces Kloeckeri Guilliermond & Peju, C. R. Soc. Biol. 82: 1343, 

 1919; Bull. Soc. Myc. France 36: 164-172, 10 pis., 1920; Konokotina & Kras- 

 silnikov, Jour. Microbiol. Leningrad 9: 93, 1929; Bachinsky, Jour. Microbiol, 

 Leningrad 9: 14, 1929. 



Originally isolated from the throat of a patient with angina. Later iso- 

 lated from fluid obtained in lumbar puncture by Bachinsky, 1929, in Leningrad. 



Cells ovoid, 2-3.5 x 3-7/^ in young malt extract cultures, with sediment and 

 ring. Asci 1-spored. heterogamous, rarely isogamous, copulation. Spores 

 spherical, verrucose, with oil droplet. 



Colony on malt agar, after 75 days at 15° C, gray yellow (Klinsieck 

 Code 103A), moist, shining, center elevated and granular, with radial folds 

 and wavy margin.' Growth on ethyl alcohol good, but organism cannot assimi- . 

 late nitrates. Slight fermentation of glucose. 



