312 MEDICAL MYCOLOGY 



Debaryomyces Nadsoni Guilliermond & Peju, Bull. Soc. Myc. France 37: 

 35-38, 1921; Rev. Med. de I'Est [Nancy] 50: 342-347, 1922. 



Isolated from a case of sycosis, probably a secondary invader. 



Cells spherical or ovoid, often united in groups consisting of a mother 

 cell and the cells sprouted from it, the older cells containing a single oil 

 globule, 2-8 X 2-6/i,. Asci arising from heterogamic copulation between a large 

 and small cell, often between mother and daughter. Usually one (rarely 

 more) ascospore per ascus, 2-3/x in diameter, wall rough, containing one oil 

 globule. At germination, the ascospores swell and lose the verrucosities of 

 the wall. ]\Iinimum temperature for sporulation 8'^-9° C, optimum 20°-25° C, 

 maximum 34°-35° C. 



Growth between 3° and 46° C, optimum 25°-30° C. On malt extract, whitish 

 deposit visible after 36 hours ; a ring formed after 5 or 6 days but no pellicle. 

 By Lindner's microfernientation method, no fermentation was observed, su- 

 crose slightly inverted. 



Debaryomyces Matruchoti Grigorakis & Peju, C. R. Soc. Biol. 85: 459-462, 

 1921. 



Isolated from feces of patient suffering from helminthiasis. 



After 24 hours at 25° C, on malt extract, cells spherical or ovoid, 3.6-5.4 

 K 1.8-3fi. In old cultures, cells usually ovoid Avitli large fat globule. On 

 Gorodkova agar, sporulation is common, usually heterogamous but occasion- 

 ally isogamous. Asci are 1-spored. Spores verrucose with large oil globule. 



On malt agar, after one month at 25° C, colony attains size of a one-franc 

 piece, white and shining, center slightly rosaceous. On Gorodkova agar, old 

 colonies are chocolate brown. In malt extract liquid, a slight sediment ap- 

 pears after 48 hours at 25° C, finally a ring, but no pellicle. On malt gelatin, 

 colony slight yellowish white, center raised, margin lobed, rays present. The 

 gelatin is not liquefied. Upper limit of growth is 37°-38° C, sporulation limit 

 30°-32°. Sucrose inverted, mannose alone fermented. Dekker (1931) claims 

 there is no fermentation at all. 



Debaryomyces Fabrii Ota, Derm. Woch. 78: 286-289, 312-316, 1924. 



Hefe Fabry, Munchen. Med. Woch. 64: 1557, 1558, 1917. 



Debaryomyces Fabryi Dekker, Verhandel. K. Akad. Wetensch. Amsterdam, 

 Afd. Natuurk. 28: 475, 1931. 



Isolated from interdigital mycosis in Dortmund. Nonpathogenic to mouse. 



On malt agar, after 24 hours at 25° C, cells spherical or ovoid, 2-6/a in 

 diameter, occasionally 8-10/i,, in groups or pairs, very rarely in chains. At 

 this time the oil drop is very small, but after 10 days the cell is larger and 

 the oil drop large. On carrot, morphology is the same as on malt agar, but 

 the oil drop is larger, and there is more ascus formation. On Gorodkova agar, 

 no mycelium, heterogamy and sometimes isogamy, ascus ovoid or polyhedral 

 with thick membrane 5-6ju. Spores ovoid or polyhedral, verrucose. Oil drop 

 colors readily with Sudan III. 



