314 MEDICAL MYCOLOGY 



inverted, slight fermentation of glucose and sucrose. Trace of fermentation 

 of fructose, galactose, mamiose reported. None with maltose, lactose, and 

 raffinose. 



Debaryomyces Lundsgaardi Ota, Ann. Parasitol. Hum. Comp. 1: 130-132, 

 1923. [Original case by Lundsgaard, Klin. Monatsh. Augenheilk. 38: 13-19, 

 1900.] 



Hefe Lundsgaard Sasakawa, Centralbl. Bakt. I, 88: 274, 1922. 

 Isolated from hypopyokeratitis in 1900. Found nonpathogenic to guinea 

 pig in 1923. 



After 24 hours at 25° C, cells spherical or ovoid, 2.5-3. 5/t, solitary or in 

 pairs, rarely in colonies, occasionally showing oil globules. In 3 days, some 

 cells are as large as 6-8/* with more abundant oil globules. In 9 days, an 

 occasional chain may be seen, but never such as in D. Hudeloi. After 40 days, 

 occasional cells as large as 12 x Idfi. On carrot, form and size of cells as on 

 malt agar, but with more giant cells, thicker membranes, and larger fat 

 globules. 8porulation is rare but heterogamous, the male cells being small and 

 elongate. Asci spherical or ovoid, 3-5/x in diameter, with thick wall. Asco- 

 spores 2-S/x, slightl}^ cchinulate, containing small oil globule. 



Giant colony attains diameter of 5 cm. in 35 days, is brilliant white with 

 fine radial rays, margin definite with rare indentations. In malt extract, a 

 brownish white sediment appears. Growth at 35° C, but not at 36° C, good 

 growth at 12° C, and some as low as 5° C. No liquefaction of malt gelatin 

 in 37 days. Organism inverts sucrose, but does not ferment any of the usual 

 sugars. 



Debaj-yomyces Laedegaardi Ota, Ann. Parasitol. Hum. Comp. 1: 132-134, 

 1923. 



Isolated from skin infection (Sasakawa). Also in Krai collection, with- 

 out record of pathogenicity. Nonpathogenic to guinea pig. 



On malt agar, after 24 hours at 25° C, cells appear ovoid, spherical or, 

 occasionally, cylindric, solitary or in groups, 2.5-5/^ in diameter. After 3 days, 

 cells a little larger. After 9 days, a few chains of long slender cells appear. 

 Asci very rare. On carrot as on malt agar, with cells in old cultures up to 

 10/x in diameter. On Gorodkova agar, in 12 days appear asci, ovoid or poly- 

 hedral with thick membrane, 3.5-4/i,, or, if oblong, 5-6/a broad. Ascospores 

 2-2.5)u,, veri'ucose, with small oil globule. Copulation heterogamous. 



Giant colony attains diameter of 3 cm. in 25 days, yellowish white with 

 fine rays from center to periphery, edge definite. In malt extract, there is a 

 brownish white deposit without pellicle or ring. No liquefaction of gelatin 

 in one month. Sucrose inverted, glucose somewhat fermented, hence sucrose 

 also. No fermentation with other sugars. 



Debaryomyces Hildegaardi Ota, Ann. Parasitol. Hum. Comp. 1: 134-136, 

 1923. 



Blastomyces Hildegaard Sasakawa, Centralbl. Bakt. I, 88: 273, 1922. 



