SACCHAROMYCETACEAE 315 



Deharyomyces Matruchoti race Hildegaardi Dekker, Verhandel. K. Akad. 

 Wetensch. Amsterdam Afd. Natuurk. 28: 471, 1931. 



Organism came from Krai collection. Nothing is known of its origin. 

 It is nonpathogenic to guinea pig. 



Grown on malt agar at 25° C, for 24 hours, it produces spherical, ovoid, 

 and sometimes elongate cells, solitary or in chains, most often in pairs. In 

 8 days, cells may be as large as 7/a in diameter. In 40 days, membrane is 

 thickened and large oil globules appear. On carrot, cells are large, up to lOju, 

 m diameter. Heterogamy usual, occasionally isogamy. On Gorodkova agar, 

 after 9 days, appear 1-spored asci. Spores are 2.5-3.5/x, uniguttulate, echinulate. 



In malt extract, a brownish white sediment appears. Organism grows at 

 36° C, but not at 37° C. Growth is fair at 12° C, but at 5° is almost absent. 

 Malt gelatin is not liquefied in 35 days. Reported to invert sucrose, and 

 slightly ferment glucose, fructose, maltose, and sucrose, but not galactose, 

 lactose, mannose, or raffinose. Dekker (1931) denies any fermentation. 



Debaryomyces Gruetzii Ota, Derm. Woch. 78: 283-289, 312-316, 1924. 



Deharyomyces Matruchoti race Gruetzii Dekker, Verhandel. K. Akad. Wet. 

 ensch. Amsterdam Afd. Natuurk. 28: 477, 1931. 



Isolated from mycosis of a nail of a six-year-old child in Kiel. Fatal to 

 mouse in 26 days. Organism recovered. 



In 24-hour-old cultures on malt agar, cells are spherical or ovoid, solitary 

 or in pairs, sometimes in groups, 2-4/a in diameter. On fourth day these have 

 increased to 7-11/a. Month-old cultures contain many polyhedral cells. Asci 

 appear in 10 days and are ovoid or polyhedral, 5-7/*. Asci appear earlier on 

 carrot. Ascospores are spherical, verrucose, 2-3/a. 



On malt extract, yellow brown sediment appears, but no ring. The or- 

 ganism was reported to ferment glucose, fructose, mannose, sucrose, but Dek- 

 ker (1931), using quantitative methods, denied this. 



Debaryomyces Guilliermondii Dekker, Verhandel. K. Akad. Wetensch. 

 Amsterdam Afd. Natuurk. II, 28: 1 : 457-459, 1931. 



Cryptococcus Fari^iae Pollacci & Turconi, nom. nud. 



Originally isolated from spoiled sausages by Cesari & Guilliermond. Re- 

 ported by Agostini (1934) from blastomycosis of the Gilchrist type (case of 

 Farina, 1929). 



Cells 3-8/x in diameter; asci 4-8/i,, 1-spored, copulation isogamous or heter- 

 ogamous ; spores 2.5-4.5/1 in diameter, spherical, rough, with a large oil drop. 



On Pollacci agar, colonies white, round, margin subsinuous, plane, be- 

 coming confluent, creamy white, minutely verrucose. On Sabouraud and malt 

 agar, growth similar but sloAver, brownish yellow on maltose agar. On carrot 

 and potato, colonies similar but Avhite and powdery. On malt gelatin, colony 

 almost smooth. In liquid media with sugars, pellicle Avhite, thick, elastic, 

 sediment yellowish white. On ethyl alcohol, pellicle thin, dull. No fermenta- 

 tion of sugars, sucrose inverted. Gelatin liquefied. 



