SACCHAROMYCETACEAE 317 



59, 1898; Ann. Ig. Sperim. 8: 229-243, Pis. 2-3, 1898; Wilhemi, Diss. Bern [Cen- 

 tralbl. Bakt. II, 4: 305-309, 353-361, 412-417, 713, 1898J. 



Atelosaccharomyces guttulaUis Beurmann & Gougerot, Bull. Mem. Soc. 

 Med. Hop. Paris III, 28: 251, 1909. 



Remak, Diagnostisehe und Patliogenische Untersuchiingen, 221-225, 1845. 



Frequently isolated from feces, especially those of guinea pig (Remak 

 1845), also from digestive tracts of rabbits. Pathogenic to both guinea pig 

 and rabbit in subcutaneous or intraperitoneal injections. 



Cells ellipsoid or oblong-ovoid, 6-8 x 15-20/t (Fig. 66, 1-4). Asci 1-4-spored 

 (Fig. 66, 5-9). Exospore ruptured on germination of spore. Spores have not 

 yet been produced on artificial media but are obtained by alternate wetting 

 and drying of the feces. 



On glucose agar, colony is thin, moist, dirty white, regular margin with 

 branching in old cultures. On glycerol a^ar, colony small, center elevated, 

 margin branched and areolate. On potato, colony is slightly elevated, dirty 

 white with elevated white points, margin also elevated, sinuous, slightly 

 powdery. In acid glucose broth, a thick, powdery white pellicle forms. Su- 

 crose inverted, glucose and sucrose fermented. On gelatin, colony circular, 

 moist, whitish, margin irregular. Gelatin not liquefied. Casein precipitated 

 in milk without acid formation. 



SACCHAROMYCES 



Saccharomyces Meyen, Arch. Naturgeschichte [Wiegemann] 4: 2: 100, 

 1837; emend. Reess, Unters, ii.d. Alkoholgarungspilze 80, 1870. 



The type species is Saccharomyces cerevisiae Meyen. 



Cells spherical, ovoid, or elongate, often in small chains, vegetative repro- 

 duction by sprouting ; in malt extract a sediment, often a ring and very rarely 

 a pellicle ; asci parthenogenetic, spores 1-4 per ascus, spherical, ovoid, rarely 

 kidney-shaped, smooth-walled ; occasionally spores copulate before germina- 

 tion ; glucose always fermented, usually also the other sugars ; nitrate not 

 assimilated; poor growth or occasionally good growth on ethyl alcohol as a 

 substrate but never producing a pellicle on this medium. 



Key to Species 



Colony rose to vermilion. *Si. granulatus. 



Colony grayish rose, grayish white on peptone gelatin. S. anginae. 



Colony yellowish, ochraceous, or brown. 



Growth only on potato, colonies ochraceous yellow with chocolate center. 

 Growth on many media. ^- Ferrani. 



Fermentation with most sugars, colony brownish gray. S. Hagiwarae. 



Lactose not fermented, colonies white at first becoming chocolate brown in one month. 

 Gelatin liquefied. S. Lemmonieri. 



Gelatin not liquefied. S. Zimmeri. 



Lactose and maltose not fermented, colony brownish. S. tumefaciens. 



No fermentation, colony yellowish becoming brown, cells often thick-walled, held, 

 together by mucous sheath. S. Blanchardi. 



