320 MEDICAL MYCOLOGY 



Colonies round, brownish gray, moist, shining, center slightly elevated, 

 with radiations at the margin. Optimum temperature 17-20° but growth pos- 

 sible between 0° and 55° C. Fermentation of maltose, dextrin, dextrose, 

 fructose, galactose, lactose, sucrose, etc. 



The figures are poorly reproduced, but those showing spores are quite 

 plausible. 



Saccharomyces tumefaciens Castellani & Chalmers, Man. Trop. Med. ed. 

 2, 768, 1913. 



Saccharomyces subcutaneus tumefaciens Curtis, Ann. Inst. Pasteur 10: 449- 

 468, 1896. 



Isolated from myxomatous tumor and lumbar abscess. Pathogenic for 

 rat, mouse, rabbit, guinea pig, and dog. 



On acid peptone broth, cells are ovoid or spherical, 3-6/i, in diameter, wall 

 thick, one to two refractive granules to a cell, no capsule. On sugar media, 

 sells larger and in chains of 3-4 cells. Spores are spherical, 1-4 per ascus. 



Colony on potato, creamy, becoming brownish. On potato and glycerol, 

 colony creamy, abundant. Growth from gelatin streak in creamy elevated 

 spots. Feeble development in broth, with light floccose sediment. No growth 

 on serum. No veil on liquid media. Glucose and sucrose fermented, not 

 lactose or maltose. Gelatin not liquefied. 



Saccharomyces Blanchardi Guiart, ? Precis Parasitol. 1910; Castellani & 

 Chalmers, Man. Trop. Med. ed. 2, 768, 1913. 



Saccharomyces sp. Blanchard, Swartz & Binot, Arch, de Parasitol. 7: 489- 

 507, 1903. 



Isolated from a gelatinous mass weighing a kilogram which was taken 

 from the peritoneum in the vicinity of the appendix. Cultures from this mass 

 gave principally this species. A rabbit, inoculated with this organism, died in 

 about a week from diarrhea, but showed no peritonitis. Masses of the yeast 

 were found in the kidneys. Also fatal to rats, mice, guinea pigs, and marmots, 

 but not to hens or pigeons. 



Cells spherical, 4-10/* rarely up to 20/a in diameter, rarely in chains, 

 often with several cells united in a common capsule whose gel is frequently 

 as thick as the cells themselves. After a week on sugar agar, asci appear. 

 These have thick membranes, are 8-spored, the spores 3/a in diameter. 



On sugar agar, at 22° C, growth slow, colony appearing after 5-6 days, 

 attaining size of a lentil after 3 weeks, and at one month diameter of only 

 2 cm., opaque yellowish white or clear gray, surface granular with irregular 

 contour, edges thick; with age, colony gradually becomes browner. On ordi- 

 nary agar, growth is scanty and even slower, otherwise similar. Growth on 

 potato slow but abundant, being several millimeters thick in a month, colony 

 yellowish white, becoming verrucose and clear brown. On carrot, colony 

 abundant and viscous. On potato glycerol, growth more rapid than on potato, 

 colony gelatinous, clear yellowish white. On gelatin plates, colony whiter, 

 more regular, round or festooned, and of a varnished appearance. Streaks 



