SACCIIAROMYCETACEAE IMPERFECTAE 355 



thin-walled, continuous, hyaline or light coJorod; no mycelium or pseudomy- 

 celium ; no endospores, young cells nni- or plui-jguttulate ; pellicle dry, chalky 

 white, often folded at tirst, becoming- smooth, citron yellow to brownish yel- 

 low, clinging to the walls, ductile, or breaking, and portions floating on the sur- 

 face. Sediment loose, often powdery. Gelatin liquefied, sugars fermented. 



Key to Species 



Ring, but no pellicle on glucose broth, colonies on potato glycerol dry, white, beginning to 



yellow about twenty-fifth day. E. fusca. 



Neither ring nor pellicle on glucose broth. 



Colony white or grayish, sediment white. E. Bernasconi. 



Colony ochraceous, sediment yellowish. E. irritans. 



Eutorula fusca (Fontoynont & Boucher) Dodge, n. comb. 



Cryptococcus fuscus Fontoynont & Boucher, Ann. Derm. Syphiligr. Yl, 4: 

 325-330, Fig. 6, 1923; Fontoynont (nomen nudum), Bull. Mem. Soc. Chirurg. 

 Paris 48: 439-442, 1922. 



Torulopsis fusca Almeida, Annaes Fac. Med. Sao Paulo 9: 10, 1933. 



Isolated from ecthyma of the leg and mycetoma of the foot, with black 

 grains (according to testimony of patient, but grains not seen by the authors). 

 Pathogenic to rat, rabbit, and pigeon. 



Yeast cells ovoid, 6-7/a in diameter, thin-walled. 



On maltose or glucose agar, colonies white, becoming cafe-au-lait in color 

 on eighth day and, finally, caramel colored. Elevated as in other slants. On 

 "poor" agar, colonies small, not elevated, beginning to brown on the fifteenth 

 day. Colonies on potato with glycerol, white and dry, 3 mm. thick, beginning 

 to yellow on the twenty-fifth day. On sweet potato, colonies thick, creamy, 

 colorless, shining, turning cafe-au-lait by the eighth day. Gas bubbles appear 

 on thirteenth day. Growth begins to yellow on twenty-fifth day and by the 

 third month turns a caramel color. On gelatin w^ith glucose, colonies thick, 

 white, creamj'. Slow liquefaction after twenty-fifth day with liquefaction com- 

 plete on the fifty-seventh day with a brown sediment. On glucose gelatin slant, 

 colony hemispheric with a thin edge, turning caramel after a time. Glucose 

 broth becomes turbid with large white sediment, ring but no pellicle. Liquid 

 clears by the fiftieth day, with ring and deposit tui'ning cafe-au-lait in color. 

 Optimum temperature for growth 20°-22° C, growth very slow at 37° C. 



Eutorula Bernasconi (Fontoynont & Boucher) Dodge, n. comb. 



Cryptococcus Bernasconi Fontoynont & Boucher, Ann. Derm. Syphiligr. 

 VI, 4: 318-325, Figs. 4-5, 1923; Fontoynont, Bull. Mem. Soc. Chirurg. Paris 48: 

 439-442, 1922 (nomen nudum). 



Torulopsis Bernasconi Almeida, Annaes Fac. Med. Sao Paulo 9: 10, 1933. 



Cause of extensive ulceration of the skin, with multiple suppurating ade- 

 nitis. Slightly pathogenic for rabbit. In pigeon secretes a soluble toxin which 

 is fatal. 



