TRICHOPHYTONEAE 483 



trary, it seems safe to assume that this species Avas present, since it is the most 

 common in the parts of Europe where the work was done. 



Inoculations into guinea pig and even man, gave negative reactions in 

 the early work. By keeping the epidermis moist, inoculation to man is easy, 

 while inoculation of guinea pig is difficult. Common in England, France, and 

 Austria, since the war of 1914-1918, endemic in Germany, United States, Brazil, 

 Argentina, occasional in Spain, Hungary, China, and Japan. 



Mycelium septate, branched, 3-6/*, mostly 3-4/*, tips swelling to form 

 closterospores either singly or in groups of five to seven ; closterospores 20-35 

 X 6-8/1, averaging 4-5 cells, cells varying from twice as long as wide to iso- 

 diametric, walls very thin, sessile or the basal cell elongate, simulating a 

 pedicel. Repeated subculture on sugar media produces the usual pleomorph- 

 ism with mycelium, 2-3/i, chlamydospores not rare, closterospores unicellular 

 and arthrosporcs becoming very rare in cultures. Sabouraud reports that 

 finally only aleurospores are produced, Grigorakis reports always finding a 

 few closterospores, while Langeron & Milochevitch report that they were un- 

 able to find aleurospores on any medium. The latter authors report spirals 

 on media prepared from cereals, dung, and dextrine + peptone. 



Colonies greenish yellow (color of partially ripe citron) small, center ele- 

 vated (suggesting a monk's hood), velvety, dry, powdery, seldom over 2 cm. 

 in diameter, the number of radial furrows increasing with age. At 37° C, 

 the colony becomes pointed and dark brown. Pleomorphism sets in in 3-4 weeks, 

 hyphae white or gray, on subculture becoming snow white. On Sabouraud 

 conservation agar, center broadly umbilicate with umbo, margin irregularly 

 elevated, citron yellow, powdery, not pleomorphic. When pleomorphic myce- 

 lium is transferred to this medium lacking carbohydrate, the colonies are 

 crackled, poor, soon dying. On Pollacci agar, colonies more greenish in color. 



Var. clypeiforme (MacCarthy) Dodge, n. comb. 



Epidermophyton clypeiforme MacCarthy, Ann. Derm. Syphiligr. VI, 6: 

 27-30, PI. 1, 1925. 



Isolated from sole of foot and between toes of a soldier. Inoculation of 

 guinea pig unsuccessful. France, also reported from Sao Paulo, Brazil. 



Hyphae S-lO/i in diameter, septa distant, only closterospores present. 



Colony on eighth or ninth day, shows a slight grayish white tuft, on the 

 eighteenth day is 2.5 cm. in diameter, central two-thirds grayish white, outer 

 fringe greenish gray; a little later the central part of the culture takes the 

 shape of a shield with a greenish yellow center covered with a velvet having 

 the appearance of chamois, the outer centimeter is flat. Colony 5 cm. in 1 

 month, 8 cm. in 2 months, color a little deeper, especially on the margins, with 

 no radial furrows. On Sabouraud conservation medium, growth about half 

 as rapid, and after 3 weeks the center is irregularly depressed. On potato, 

 less characteristic, a little less velvety, green color slightly more marked than 

 on other media. 



