TRICHOPHYTONEAE 485 



The culture assumes a rose tone, becoming salmon on the twelfth day, with 

 a deeper fire-colored center. Pleomorphism begins on the sixteenth day. When 

 first isolated, the colony is purplish and turns the medium slightly purple. On 

 Sabouraud conservation agar with peptone 4%, colony moist, shining, pearl 

 gray, velvety, rounded and radiating, on the eighth day becoming darker, 

 almost brownish. On carrot, velvety, rose after first day, salmon fourth day, 

 and pleomorphic on the tenth day. Similar on potato, but on tenth day, 

 colony is rose-salmon, crateriform with a cerebriform margin. Colony di- 

 ameter 1 cm. in 24 hours, 2 cm. in 48 hours, 3 cm. in 4 days, 5 cm. in 9 days, 

 7 cm. in 11 days. 



Epidermophyton purpureum (Bang) Dodge, n. comb. 



Trichophyton purpureum Bang, Ann. Derm. Syphiligr. V, 1 : 224-238, Figs. 

 1-7, 1910. 



Isolated from tinea cruris, spreading to thighs, buttocks, scrotum but net 

 to pubis. One patient, a Dane, acquired the disease in America, the other 

 patient, a Mexican; lesions healed by pomade containing 1% chrysarobin. 

 Harris & Lewis (1930) report a case of subcutaneous granuloma from which 

 this organism was isolated. Probably cases from southern United States, 

 Porto Rico, the states of Amazonas and Sao Paulo in Brazil, Uruguay, and 

 Buenos Aires, Argentina belong here, although they usually appear under the 

 name E. ruhrum in the literature. Inoculation of guinea pig and rabbit by 

 a harsh shave and rubbing in the mycelium and spores from the culture. 

 Lesion develops on the eleventh day and disappears in the fourth week, or- 

 ganism recovered. 



Mycelium 2-3 cm. in diameter; cells 2.5 times as long as thick, when 

 young, becoming isodiametric when old ; aleurospores small, 4-5 x 2.5/li, pyri- 

 form, on compound thyrses on the eighth or ninth day; closterospores on the 

 tenth to twelfth day, 4-8 celled as in Ectotrichophyton mentagrophytes, borne 

 both terminally and laterally; chlamydospores occurring abundantly in old 

 cultures. 



Growth evident on sixth to eighth day, white, size of a 50 centime piece 

 in 10 days ; colony hemispheric with long white velvet ; on the tenth to twelfth 

 day, the lower portion of the colony becomes red purple which gradually ex- 

 tends upward ; on the twentieth day, the colony has a white center surrounded 

 by a powdery zone, 5-6 mm. broad, neutral in color, due to the pigment forma- 

 tion below. On Sabouraud glucose, growth slower, slight radial folds near 

 the margin, purple pigment not diffusing into the medium ; reverse purple. 

 On Sabouraud conservation agar with 3% peptone, on twenty-fifth day, colony 

 hemispheric, grayish white ; on fortieth day, 5 cm. in diameter, center covered 

 by white velvet, elevated, surrounded by circular furrow with radial folds, 

 outer zone gray or slightly bister and powdery with margin 5 mm. broad, 

 slightly velvety and white ; reverse margin not colored. 



Very old cultures fail to produce the typical center, but inoculation into 

 and reisolation from guinea pig renews this power. 



