ASPERGILLACEAE 627 



bars of orange yellow coloring matter deposited under the loose outer wall 

 upon the firm inner wall. ISclerotia occasionally produced, usually purple or 

 reddish ])uri)le, globose to pyriform with apex white." 



Aspergillus Gratioti Sartory, C. R. Acad. Sci. 170: 523, 524, 1920. 



Found on the nails of a Chinese workman in France. 



Pieces of the nail, on being treated with 40% KOH, show sometimes sep- 

 tate mycelium, sometimes unsegmented. Chlamydospores 20-40;u, in diameter, 

 sometimes terminal, frequently intercalary. Conidia free, 3.5-4^ in diameter. 

 In culture, hyphae grayish, then brown and black, 0.6-1. 5/i, in diameter, much 

 branched. Fertile hyphae short with delicate walls about 4-5ju, in diameter, 

 vesicle spherical, 8-20/a in greatest diameter. Phialides elliptic, 6/a long, very 

 close-packed. Conidia globose, 3-3. 5/x in diameter, brown. Sclerotia and peri- 

 thecia not seen. 



On Raulin's maltose agar, colony is moist and grayish, becoming visible 

 on the fifth day, black by the tenth. Colony elevated, irregularly rugose in 

 the center, less so at the margin. Conidiophores in concentric circles. Seen 

 from below, culture appears brown with the substrate also colored. No marked 

 odor. Growth on glucose agar the same. Growth the same, but less pronounced 

 on other media; e.g., potato, potato glycerol, carrot, Sabouraud's nutrient gel- 

 atin, a variety of Raulin's sugar solutions (sucrose, glucose, lactose, maltose), 

 and milk. No growth on coagulated beef serum, egg albumen, or acid potato. 

 Milk coagulated on the twelfth day, curd digested. Gelatin liquefied on the 

 fifth day. 



Aspergillus subfuscus Johan-Olsen [Sopp] & Gade, Nord. ^led. Ark. 18: 

 9:14-16, 1886; Johan-Olsen [Sopp], Bot. Jahresber. 14: 476, 1886; Meddel. 

 Naturhist. Foren. Kristiania, 1885. 



Sterigmatocystis suhfu.^ca, Saccardo, Syll. Fung. 10: 526. 1892; Sartory & 

 Jourdre, C. R. Soc. Biol. 64: 928, 1908. 



Pathogenic when injected into rabbits. 



Mycelium snow-white, coarsely articulate, sparingly branched. Curious 

 raylike involution forms present, conidiophore 18-20/x from base to vesicle, 

 vesicle 30-90/a, head, including chains of conidia, 90-200/a in diameter. Phialides 

 20/i long, conidia 3-3.5/* in diameter, olive brown, spherical, smooth. At opti- 

 mum temperature of 36-38° C, conidia appear in 30-36 hours, more slowly at 

 lower temperatures. No perithecia or sclerotia reported. 



Organism grows best on bread medium (10 c.c. finely crumbed bread. 

 125 c.c. H2O, and 2 drops lemon juice), also groAvs on agars and gelatin. Giant 

 cultures, grown at room temperature, are olive brown in color. At 37° C, 

 color deeper, then greener. Mycelium white, quite smooth then raised above 

 the substrate. Colony lightest at the edges, deepest in color at center. 



Aspergillus cinnamominus (Weiss) Dodge, n. comb. 



Sterigmatucystis cinnamuminus Weiss, Ann. Parasitol. Hum. Comp. 8: 189- 

 193, 5 iigs., 1930. 



