TORULEAE 675 



though growth is good in media containing glucose, fructose, maltose, dextrin, 

 lactose, 2% methyl or ethyl alcohol, citric, malic, tartaric or acetic acid. 

 Peptone, asparagin, glycocoll, ammonium sulphate or potassium nitrite or 

 nitrate may be utilized as sources of nitrogen. 



Cryptococcus metaniger Castellani, Arch. Derm. Syphilol. 16: 402, 403, 

 1927; Amer. Jour. Trop. Med. 8: 393, 1928, probably also belongs here. It was 

 isolated from a case of trichomycosis nigra and described as cells elongate, 

 black, colonies black on all laboratory media. Too vaguely described for 

 definite placing by any one at all familiar with this large group. Ciferri, 

 Arch. Protistenk. 71: 445, 446, 1930, has also placed it here, while Ferrari 

 (1932) places it in Cladosporium. 



Pullularia Jeanselmei (Langeron) Dodge, n. comb. 



Torula Jeanselmei Langeron, Ann. Parasitol. Hum. Comp. 6: 385-403, 12 

 figs., 1928. 



Fig. 107. — Pullularia Jeanselmei. (After Langeron 1928.) 



Isolated from black grain mycetoma in Paris in a mulatto woman from 

 Martinique. Case history by Jeanselm, Huet & Lotte, 1928. 



Mycelium in lesions forms grains 500-1,000/x in diameter, soft, irregular, 

 and appearing like hollow cylinders. Hyphae coiled, interstitial gel not well 

 developed. Hyphae, 2.5-3/* in diameter, forming chains of arthrospores, 4.5-5/i, 

 which soon disintegrate (Fig. 107). 



In cultures, the arthrospores form chains of spherical cells which laterally 

 produce ovoid blastospores in the vicinity of the septa. Toward edges of the 

 colony, fewer lateral chlamydospores are produced and terminal whorls like 

 conidia are produced on short lateral branches. These chlamydospores are 

 2.5 X 5yu,, often with 3-8 per group. On sugar media contours are rounded and 

 submoniliform. Cultures grow well at 22°-25° C. on the usual media (Sabour- 

 aud glucose and malt agars, potato-glycerol, carrot and potato decoctions). 



