TORUIiEAE 683 



is similar to that on potato but more rapid. lu hay infusion, there are cottony 

 tufts with slight sediment and no clouding of the medium. 



Madurella Tabarkae Blanc & Brun, Bull. Soc. Path. Exot. 12: 741-748, 

 1 pi, 1919. 



Isolated from a black grain mycetoma which was subcutaneous without 

 involving muscles or bones. Grains removed and wounds healed normally. 



The sclerotia are composed of fine filaments, 2-4/a in diameter, with chlamy- 

 dospores either terminal or intercalary, 12/x in diameter. Hyphae slender, 

 cells short. Optimum temperature 22° C, growth slow at 35° C. 



Organism grows best on Sabouraud maltose agar. On simple Sabouraud 

 agar, it produces good colonies, with the growth finally becoming rounded 

 and folded. Color begins at the center and spreads outward. After one 

 month, center is copper yellow and periphery greenish yellow, with amber 

 3'ellow guttation in the center. Culture finally becomes covered with a brown 

 pubescence and medium turns brown. Similar, but faster, on Sabouraud 

 maltose agar. On ordinary agar, hay infusion, or potato agar, in 24 hours, 

 small white colonies appear, while medium darkens progressively. Colonies 

 are never large. Similar on lead agar with growth sparse and white. On 

 neutral red agar, growth good with no change or browning of medium. On 

 potato, potato glycerol, or carrot, small white colonies which become yellow 

 and folded; potato rapidly blackens. On coagulated serum, growth as an 

 ordinary agar except that colony is rose at first, later becoming brown. On 

 gelatin stab, there is abundant growth on the surface, which becomes in- 

 tensely brown with arborization and sclerotium formation below. In beef 

 peptone broth in 24-48 hours, small globular masses are especially abundant 

 in contact with the tube. Medium progressively browned up to the fifteenth 

 day. In peptone broth, growth is the same. No indol formation. No growth 

 in hay infusion. In potato infusion as in beef broth, except that the floecu- 

 lent colonies slowly collect at the bottom of the tube. Black sclerotia appear 

 in about 25-30 days. In peptone sugar solutions, neither acid nor gas is 

 formed (no fermentation). Growth as in beef broth. In peptone bile, small 

 spherical colonies appear with browning of the medium. Milk is coagulated 

 on the eighth day with browning of the medium and abundant growth on the 

 surface. Gelatin not liquefied. 



Madurella Ikedae Gammel, Arch. Derm. Syphilol. 15: 241-284, 20 figs., 

 1927. 



Isolated in Minneapolis by Ikeda from a black grain maduromycosis. 

 Nonpathogenic to rabbit or guinea pig. 



Mycelium grayish white, later light brown, darkening certain sugar media. 

 Hyphae hyaline or subhyaline, rarely granular, varying in diameter from 

 1.5-5/i. Chlamydospores numerous only in Sabouraud glucose broth. In all 

 liquid media many hyphae break up into chains of citriform or spherical 

 spores. Acuminate light brown colonies on both Sabouraud and Griitz a gars. 

 Sclerotia numerous on surface and in depth of Sabouraud preservation me- 



