TRICHOPHYTONEAE 547 



Producing tinea capitis microsporica in Japan; also isolated occasionally 

 from cases of trichophytia maculosa and kerion Celsi. [Literature unknown 

 to me earlier than Kambayashi (1932) which does not mention pathogenicity.] 



In cultures, hyphae straight or undulate, branching dichotomous, 2-3.5/x 

 in diameter; aleurospores rare, 2-3. 5/a, ovoid or pyriform; closterospores 3-4 

 locular, rare, degenerate, and not well differentiated; chlamydospores abun- 

 dant, terminal, or intercalary, usually ovoid, variable in size from 4-19/*; 

 arthrospores in very old cultures as also forms suggesting short favic cande- 

 labra and other densely branching aggregates. 



Tlie interpretation of the possible sexual state figured by Kambayashi 

 (1932) is extremely difficult. A terminal cell suggesting a young chlamydo- 

 spore is apparently the female organ. The penultimate cell grows out as a 

 branch, suggesting an antheridium, M^hich grows up the side and fuses with 

 the female cell at the tip. In other cases the penultimate cell is said to invade 

 the terminal cell, Avhich degenerates, and to produce a coil of cells within the 

 wall of the terminal cell. Asci spherical, containing 2-12 spores. Should the 

 coil of cells Avithin the ascogonial wall be considered as an ascogenou^ hypha 

 which invades the old ascogonium and produces a chain of asci, or are these 

 coils really outside the old ascogonial wall and represent the beginnings of 

 perithecia as in other Gymnoascaceae ? Or is it a wholly asexual phenomenon 

 in which a terminal chlamydospore dies and is invaded by a living cell just 

 below? While the number of spores within the ascus varies from 2-12, the 

 nuclear content seems to be in multiples of 8 and evidently the other nuclei 

 degenerate during spore formation. 



On Sabouraud maltose agar, growth very slow, colonies punctiform, 

 hemispheric, straw to citron yellow becoming knotted, elevated, and only 3-4 

 mm. in diameter after 86 days, with delicate arborescent yellowish growths 

 into the medium. Another type of colony on the same medium produced by 

 other strains has faster growth, becoming 6 mm. in 40 days, colony fiat, with 

 a slightly elevated brownish yellow to chocolate brown center and a surface 

 with irregular, more or less radial furrows, dry, brownish yellow with a red- 

 dish tone, and with yellowish arborescent outgrowths into the medium at the 

 flat margins. Later both types become identical in their giant colonies which 

 are 3.5-5 cm. in 40 days, center brownish yellow to chocolate brown, moist, 

 more or less cerebriform with very numerous, very irregular, radial folds, 

 the color mostly straw yellow to brownish yellow, the reddish tone being 

 stronger toward the center and lighter toward the margin which is almost 

 white with yellowish arborescences into the medium. 



Microsporum aureum Takeya, Tohoku Jour. Exp. Med. 6: 80-93, 1925. 



Producing tinea tonsurans microsporica in Japan (isolated in 97% of 

 Takeya 's cases). Not inoculable into guinea pig. 



Hyphae 2-3/a in diameter; chlamydospores 6-8/t; aleurospores rare. 



Colony at one month 2-3 cm. in diameter, citron yellow to yellowish brown, 

 darkening in age, surface smooth, moist, waxy, shining, never powdery or 

 velvety, not folded, marginal rays suggesting Ectotrichophyton mentagrophytes 



