ACTINOMYCETEAE 717 



fast bacilli, 0.3-0.5 x 1-4/a; rods allantoic!, mostly curved, stain unevenly. No 

 clavate forms, gram-negative ; in subcultures, gram-positive ; dots in the gram- 

 negative rods. 



Aerobic culture produces oidial form (bacilliform) in sterile ovarian fluid, 

 development with smell of old boot leather or of a tannery, as in patient's ear. 

 Not acid-fast. Grayish, tough, slimy growth. Finally, after 7 months in broth, 

 oidal converted to arthroidal form; colony spheroidal. Mycelium transferred 

 into glucose agar shake yielded surface growth, gram-negative nonacid-fast, 

 bacilliform hyphae and terminal or intercalary chlamydospores. 



On glycerol potato, oidial form shows no growth, arthroidal form thin, 

 scanty, yellow, discoloring potato and liquid. On gelatin, no growth. On 

 glucose agar shake, oidial form shows no growth in depth, arthroidal form 

 profuse, dirty yellow, slimy growth. On blood agar, oidial form solid, heaped 

 up, discrete, pale orange, no digestion of hemoglobin. Arthroidal form dirtj 

 yellow, slimy, collecting in the water of condensation as oily balls, hemoglobir 

 digested. In litmus milk, fine fawn-colored flocculus in the oidial form, coloi 

 partly dissipated with slight digestion of curd, arthroidal form showing com 

 plete decolorization and more marked digestion. In inspissated sheep serum 

 the oidial form shows slight digestion, forming a clear fluid with a slighl 

 scum, while the arthroidal form shows more marked digestion, opalesceni 

 fawn-colored fluid with a slight dirty yellow scum. 



The following organism belongs in this group, but in the absence of cul 

 tural characters, it cannot be placed more definitely. 



Actinomyces anaerobies Plant. 



Actinomyces sp. Butterfield, Jour. Infect. Dis. 2: 421-430, 1 pi., 1905. 



Oospora anaerobies Sartory, Champ. Paras. Homme Anim. 830-832, 1923 



Isolated from the lung of an American male, 19 years old, primarily suf 

 fering from diabetes mellitus. Urine showed 3.5% to 8% glucose, also acetone 

 and diacetic acid. Filaments of Actinomyces isolated from pus in wall cavity 

 of right lung, also from bronchopneumonic tissue constituting the walls of the 

 cavity. In the latter, the organism was present in the small bronchi, bron- 

 chioles, and in the air sacs filled with leucocytes. Best demonstrated by stain- 

 ing with Weigert's modification of Gram's method. No other organisms found 



In tissues, organism usually appears as tangled masses of branching hyphae, 

 sometimes in a distinct radiate arrangement with beaded hyphae in the more 

 central portions. No clavate forms observed in examination of hundreds of 

 such colonies. Organism acid-fast. 



Actinomyces viridis (Lombardo-Pellegrino) Dodge, n. comb. 



Streptothrix viridis Lombardo-Pellegrino, Riforma Med. 19: 1065-1068 

 1903. 



Isolated from soil, found pathogenic to rabbit, guinea pig, and cat on ex- 

 perimental inoculation. 



Hyphae long, slender, flexuous, branched, acid-fast, aerobic or anaerobic 



