ACTINOMYCETEAE 721 



7-8 days with increasingly alkaline reaction, ammonia produced. Glucose and 

 fructose acidified but not fermented in 48 hours, glycerol more slowly. Little 

 acidity with galactose and usual disaceharides, polysaccharides, pentoses, 

 higher alcohols, and glucosides. Urea developed an alkaline reaction. No 

 nitrate reduction. Protease active only in alkaline medium producing am- 

 monia, no action on coagulated blood serum, egg white or fibrin. 



Actinomyces mexicanus Boyd & Crutchfield, Amer. Jour. Trop. Med. 1: 

 268-282, Figs. 15-18, 1921. 



Nocardia mexicana Ota, Jap. Jour. Derm. Urol. 28: [4], 1928. 



Isolated from mycetoma of the foot. Nonpathogenic to guinea pig. 



Hyphae 0.7/x, in diameter, tortuous, branching, tips sometimes slightly 

 swollen. Chlamydospores formed in blood agar and Loefller's blood serum. 

 Organism aerobic, optimum temperature being 37° C. Gram-positive, not 

 acid-fast. 



On glycerol agar, growth very slow, colonies small, 1 mm. in diameter, 

 more or less convex, tending to be wrinkled. On blood agar, colonies discrete, 

 irregular, rectangular, 3-4 mm. on a diagonal, margins abruptly elevated 1-2 

 mm., center umbilicate, efflorescent, with a narrow zone of hemolysis. On 

 Conn's synthetic citrate agar (citric acid 5 gm., NH^Cl 0.5 gm., KgHPO^ 0.5 

 gm., glycerol 6 c.c, agar 15 gm., water 1,000 c.c), colonies small, white, opaque 

 convex, moist. On Waksman's modification of Czapek's agar, no growth 

 visible. On meat infusion agar, colonies discrete, 2 cm. in diameter, with 

 abrupt edges, 1-2 mm. high, center pointed or umbilicate, smooth, moist, 

 slightly yellow. On Krainsky medium (glucose 10 gm., NH^Cl 0.5 gm., as- 

 paragin 1.5 gm., agar 15 gm., water 1,000 c.c), colonies discrete, circular, not 

 over 2 mm. in diameter, slightly convex, moist and white, mycelium penetrat- 

 ing 2-3 mm. Growth on potato very slow, white and flat, with substrate un- 

 altered. Growth also slow on gelatin. ]Meat infusion broth shows a flaky 

 sediment, with liquid remaining clear. In carbohydrate peptone water, no 

 fermentation, with colonies appearing as small white balls, not over 1 mm. in 

 diameter, in the bottom of the tube. Medium remains clear. In litmus milk, 

 growth is slow, no coagulation, digested in 3 weeks. Growth also very slow 

 in Loeffler's blood serum as discrete, rectangular colonies 2-3 mm. in diam- 

 eter, conical, center elevated 1-2 mm., colorless, moist, smooth, no liquefaction 

 in 3 weeks. Gelatin liquefied. Faint fecal odor in cultures. 



Actinomyces Rivierei (Verdun) Brumpt, Precis Parasitol. ed. 4, 1201, 1927. 



Streptothrix sp. Riviere, Cong. Franc. Med. Bordeaux 1895, 2: 1003-1011, 

 1896. 



Actinomyces Sabrazes et Rivieri Berestnev, Diss. iMoskva 1897. 



Nocardia Rivierei Verdun, Precis Parasitol. 1912. 



Discomyces Rivierei Neveu-Lemaire. Precis Parasitol. Hum. 43, 1921, non 

 Nocardia indica Kanthack fide Froilano de Mello & Pais, 1918. 



Oospora Rivierei Sartory, Champ. Paras. Homme Anim. 792, 1923. 



