722 MEDICAL MYCOLOGY 



Isolated from sputum and pus of a bronchopleuropuluionaiy infection fol- 

 lowed by multiple miliary subcutaneous abscesses. Early symptoms those of 

 tuberculosis, but Mycobacterium tuberculosis absent. Found pathogenic to lab- 

 oratory animals by the use of a specialized technic. 



Hyphae Iju, in diameter, arthrospores 1.5/i, long by 1/x in diameter. Or- 

 ganism a strict aerobe. 



Colonies on peptone agar small, conical, 1 mm. in diameter, powdery, 

 growing to about 5 mm. in diameter, reverse yellow, medium blackened. On 

 potato, growth slow, colonies appearing like little blocks of powder. Growth 

 also slow on eg^ white, the substrate not digested. In liquid media, small 

 grains size of a millet seed settling to the bottom, colonies floating, if not dis- 

 turbed. Growth good on milk, forming a flesh-colored, whitish powdery pel- 

 licle with furrows. Fat assimilated, casein and lactose not perceptibly at- 

 tacked, apparently only glycerol used from fat. Grown on 6% glycerol pep- 

 tone agar, colony mammillate. white, powdery, furrowed, cork color, finally 

 becoming black. Lactose, maltose, glucose, and fi-uctose utilized. Gelatin 

 liquefied. 



Actinomyces Gibsoni Dodge, n. sp. 



Streptothrix sjj. Gibson, Jour. Path. Baet. 23: 357, 358, 1920. 



Oospora sp. Sartory, Champ. Paras. Homme Anim. 776, 1923. 



Isolated from enlarged spleen. Nonpathogenic to guinea pigs, rabbits, and 

 mice, pathogenic to Macacus monkej's, organism being reisolated in one case 

 from an enlarged spleen. 



Gram-positive ; aerobic. 



Colonies on agar slants discrete, buff or white, hemispheric, becoming de- 

 pressed and umbilicate. White efflorescence and musty odor. In peptone 

 broth, there is a bottom growth of small, white "snowballs," with some even- 

 tually rising and floating on the clear medium. Neutral red lactose broth is 

 unchanged. Milk coagulated in 4-5 days. Gelatin slowly liquefied with growth 

 near surface. 



Actinomyces Guegueni (Ota) Brunipt, Precis Parasitol. ed. 3, 1921; ed. 4, 

 1191, 1927. 



Oospora lingualis Gueguen, C. K. Soc. Biol. 64: 852-854, 1908; Arch, de 

 Parasitol. 12: 337-360, 31 figs., 1909. 



Discomyces lingualis Brumpt, Precis Parasitol. ed. 1, 1910. 



Nocardia li^igualis Castellani & Chalmers, Man. Trop. Med. ed. 2, 819, 1913. 



Nocardia Guegueni Ota, Jap. Jour. Derm. Urol. 28: [4], 1928, excl. syn. 



Black pilose tongue by Gueguen along with Cryptococcus linguae-pilosae 

 which later he thought to be the agent of the disease. Pathogenic for guinea pig. 



Mycelium slender, spirals present, sometimes terminating in a chlamydo- 

 spore. Chlamydospores commonly intercalary, ovoid, 1-3/x in diameter, conidia 

 in chains, spherical, O.Sfi, on simple clavate conidiophores, 4-5/x. Optimum tem- 

 perature 37° C, partial anaerobe. 



