ACTINOMYCETEAE 727 



Colonies, on ISabouraud glucose agar, small, round, elevated, uneven, mam- 

 millate, snoAv white, dry, chalky, hard, crushing with difficulty. Subcultures 

 gradually die out on this medium. On Langeron 8% sugar agar, very old 

 colonies show a slight rose color. On simple agar, colony whitish, granular, 

 humid, adherent, older colonies becoming folded and eerebriform, covering 

 the surface of the agar and being somewhat sunken in the agar at the mar- 

 gins. An efflorescence appears on the third day, with greater elevation. On 

 potato, with or without glycerol, colonies become confluent, forming whitish 

 cordons, sometimes sinuous, the chalky surface mammillate and uneven 

 covering the whole surface after several subcultures, turning yellowish in 

 age with the substrate becoming greenish. On carrot, colonies creamy, slightly 

 yellowish, later becoming chalky and wiiite with carrot browning. In potato 

 juice* a fine white powder appears at the surface with white floating crateri- 

 form colonies. These are dry, 0.2-0.25, up to 0.5 mm. in diameter. Later, 

 center of crater is elevated, yellowish, waxy, finally confluent, forming a 

 waxy, thick, yellowish folded pellicle with a fine white efflorescence. In ear- 

 rot juice, prepared as the first method for potato juice,* there is growth both 

 in depths and at the surface. Medium remains clear, colonies grayish Avhite, 

 not dry. Ring prominent, 6-7 mm. above the liquid. Pellicle is thick, folded, 

 and gray with a white efflorescence, lower surface being black and liquid 

 amber. In broth, growth is somewhat as in carrot juice, but no pellicle forms, 

 and ring is not very adherent. Starch medium is liquefied. No fermentation 

 of glucose, fructose, maltose, sucrose, or lactose. Serum is completely lique- 

 fied. Gelatin also liquefied with the formation of a golden yellow pellicle. 

 Odor of cultures moldy. 



Actinomyces Dassonvillei (Liegard & Landrieu) Brumpt, Precis Parasitol. 

 ed. 4, 1191, 1927. 



Nocardia Dassonvillei Liegard & Landrieu, Ann. d 'Occulistique 46: 418- 

 426, 1911. 



Discomyces Dassonvillei Neveu-Lemaire, Precis Parasitol. Hum. 42, 1921. 



ISfreptothrix Foersteri Gasperini, Ann. Microgr. 2: 449-474, Pis. 5-7, 1890, 

 non aliorum. 



Originally isolated from a case of conjunctivitis. Potron (1913) states 

 that this species is pathogenic for man and rabbits. Since I have not been 

 able to locate the original description, the following is taken from the paper 

 by Potron & Thiry (1913). 



Hyphae 0.5-0.7/a in diameter, branched, septa not seen. Arthrospores 

 ovoid, in chains, not staining, highly refringent. 



On solid media, colonies acuminate, white, dull, chalky, very difficult to 

 separate from contaminating bacteria. On potato, colonies white, soon chalky, 

 covering the whole surface of the medium, which is colored violaceous at first. 

 Colonies become deep green {vert foncc), with guttation of greenish drops 



*Piit 250 pm. grratc-d potato in 300 c.c. water, triturate, decant, and filter. Sterilize at 

 H5°-120° C. Alternate method; cover pulp with water, autoclave 15 minutes at 115*-iaO* C. 

 decant, filter, and .^iterilize as above. 



