840 MEDICAL MYCOLOGY 



Var. Peperei Sartory, Champ. Paras. Homme Aijim. 681, 682, 1922. 



Monosporium sclerotiale Pepere, A. Soc. fr i Cultori di Sci. Med. e Natural! 

 i Cagliari 18 juin 1914; Sperimentale 68: 543, 1914. 



Scedosporium sclerotiale Brumpt, Precis Parasitol. 1114, 1922. 



Isolated from a black grain mycetoma, grains 1-2 mm. in diameter. Path- 

 ogenic to laboratory animals. 



Hyphae slightly brownish, rarely septate, 3-4/a, some 5-5. 5/x, branched, 

 sometimes united into strands. Conidiophores slender and short, erect or de- 

 cumbent, bearing a single terminal conidium, 10-14 x 4-7/1,, sometimes [young- 

 er?], spherical or ovoid and smaller, 5-7/a, point of insertion yellowish; conidia 

 with oil globules and opaque brown granules. Very rarely in old cultures, 

 2-3 spores appear at the end of a single conidiophore. Sclerotia common in 

 old cultures. Growth under anaerobic conditions very slow. In very old 

 drying out cultures, anaerobic cultures and colonies in collodion sacs in the 

 peritoneum or under the skin of rabbit or guinea pig, sclerotioid growths are 

 produced. Hyphal cells swollen somewhat irregular, contorted, having a pseudo- 

 parenchyma without and more normal hyphae within; conidiophores abnor- 

 mal, spores nearly round. Also coremia present at the periphery of the colony. 



On simple agar, colonies develop sloAvly, transparent, then white, slightly 

 elevated, confluent. On Sabouraud agar, growth good on either glucose or 

 maltose, colony round, center hemispheric, translucent or glassy, finally cov- 

 ered with hyphae. Central elevation becomes irregular, mammillate, sur- 

 rounded by a ridge, giving a somewhat crateriform appearance and ridge 

 surrounded by a furrow, not blackening. On potato or potato glycerol, small 

 white colonies with rapid groAvth, hemispheric with long cottony hyphae, in 

 6-7 days surface covered with a gray or gray brown coating with tendency to 

 greenish or black. Old colonies completely convoluted, losing their cottony 

 appearance. On beets, colonies white, much the appearance of potato. On 

 banana, growth slow, colonies umbilicate, remaining white, although in old 

 cultures brown guttation may appear. On gelatin, development not very 

 good at 20° C. ; liquefaction slow and scarce. On serum or serum glycerol, 

 colonies oval, central portion mammillate, hemispheric, translucent, glassy. In 

 broth, with glycerol or glucose, colonies translucent, yellowish white, opaque, 

 adherent, confluent, folded, then brownish; filaments from the margin climb 

 the walls of the tube. On hay infusion + agar 2%, development rapid, colo- 

 nies round, convex, with rapid formation of hyphae. On hay infusion, colonies 

 spherical, mucilaginous, center dark, finally producing a white pulverulent 

 pellicle which easily breaks up and falls to the bottom. On potato decoction, 

 about the same. In beef broth, development slow, small colonies yellowish, 

 tending to brown, surface granular, finely mammillate, adherent to the tube, 

 with aerial colonies convex, white, or grayish white. In old cultures (30-35 

 days), colonies form a long, blackish cylinder, finally reaching the bottom of 

 the tube, broth clear. Presence of sugars does not modify development. On 

 peptone water, development slow, small white colonies adherent to the walls 

 and finally making a thin white pellicle. 



