SPOROTRICHEAE 803 



Found in lesions on the tongue of an otherwise healthy ten-year-old girl. 

 First ulcer on tongue, then on hard and soft palates, and on tonsils. Some 

 lesions were small, hard, pinhead size, grayish white, reddish below, removed 

 by platinum loop with difficulty but without causing pain. Other lesions, on 

 tonsils and hard palate, apparently derived by softening of nodules, were flat, 

 shallow, ovoid, with irregular edges. Glands not swollen, temperature nor- 

 mal. The patient had returned from the Soviet Republic only a short while 

 before. Patient seen only once, since mother refused to permit hospitalization 

 or further treatment. Pathogenic to white rats. 



In the nodules, cells spherical or ovoid, 2-3 x 6-7/a. Hypliae short, branched, 

 terminal cells swollen. Gram-positive, old hyphae not staining, acid-fast. 

 Some are partly gram-positive and partly negative. In cultures, sterile and 

 fertile cells grow along side of hyphae, sessile or in fine sterigmata, either 

 singly or in groups. Chlamydospores present. 



This organism grows well on a variety of liquid or solid media either with 

 or without sugar, aerobically or anaerobically, between 18° and 37° C. On 

 Sabouraud agar, colony circular or slightly oval, silvery white, dull, slowly 

 becoming convex, elevated above the surface of the agar with a delicate, vel- 

 vety margin, growing at an irregular rate so that it finally becomes cerebri- 

 form. Reverse concolorous even after 10 weeks. Growth on potato rather 

 poorer than is the case with other species, but growth good in water of con- 

 densation. On Loeffler's medium, growth good and characteristic, center colo- 

 nies being surrounded by an aureole with rays like icicles. On gelatin, either 

 with or without sugar, growth good, colony becoming crateriform with a wide 

 rim, finally, as the medium liquefies, reaching the bottom. No characteristic 

 growth on blood agar, no hemolysis. In common broth, tiny surface colonies 

 appear after 24 hours. These settle to the bottom on shaking. Odor nauseat- 

 ing on standing. Growth still good in broth covered with paraffin. Milk is 

 coagulated in 6 days, with sediment and turbidity of the medium. 



Pleomorphisni. — At room temperature (more slowly at 35°-37° C), the 

 center of colony becomes covered with rugose coremia, destroying the appear- 

 ance of the colony. At thirty-fourth day, colony rounded, with or without 

 development below the surface of the medium, colorless at first, later becom- 

 ing pearly, retaining aureole, adherent. Appearance changes on subcultures. 

 Yeast forms are produced on many different media when temperature is 

 changed. Colonies consisting of yeast cells small, flat, oval, brownish white, 

 slimy. 



Sporotrichum equi, Carougeau, Jour. Med. Vet. Zootechnie 60: 8-22; 

 75-90; 148-153, 1909. 



Epizootic on horses in Madagascar, symptoms similar to those caused by 

 Zymonema farciminosum, with which it since has sometimes been confused. 



Thought by Hyde & Davis, Jour. Cutan. Dis. 28: 321-352, Pis. 36^5, 1910, 

 to be identical with S. Schencki as in America, organisms in horse and man are 

 identical. It is distinct from Zymonema farciminosum. 



