744 MEDICAL MYCOLOGY 



with rounded, projecting points. If the streak is so made that the colonies 

 do not become confluent, they give the typical mammillate appearance. On 

 potato, growth is slow and limited, growth only detectable by dampness of 

 the surface of the potato. Later a dry, linear growth with even margins. In 

 broth, at first a slight cloudiness with small amount of pulverulent deposit, 

 medium later clearing with a granular sediment, granules becoming flocculent 

 in old cultures. In 10-day-old broth cultures, there is a tendency to pellicle 

 formation with a fragmentary surface covering. In any case, a ring forms with 

 a dirty white color, later turning yellowish, easily broken up. No indol forma- 

 tion. No fermentation with any sugars. Milk not coagulated, gelatin gradually 

 liquefied. 



Organism grows between 18° and 42° C, optimum temperature being 

 37° C. 



Actinomyces phenotolerans C. H. Werkman in Gammel, Arch. Derm. 

 Syphilol. 29: 286-297, 1934. 



Isolated from granuloma in man. Pathogenic for guinea pigs when in- 

 oculated into the lungs. 



Mycelium straight with lateral branching, less than 1^ in diameter, chains 

 of conidia abundant, prostrate, racemose type without helices, very acid-fast, 

 hyphae gram-positive, spores gram-negative. 



On Krainsky's glucose agar, colonies small, round, adherent to medium; 

 surface powdery, white to grayish white, becoming orange in old cultures; 

 no odor, medium not discolored. On potato, colonies small, chalky, confluent 

 into orange membrane with odor of market garden soil. On gelatin, white 

 surface growth with very slow liquefaction. On serum, cream colored surface 

 growth with arborization. In Dunham peptone broth, an abundant flaky sedi- 

 ment. Good growth in broth with 0.5 per cent phenol. On sugar broths, 

 growth good with most sugars, sediment flaky with solution becoming- 

 alkaline. Litmus broth becomes alkaline with surface growth. No amylase 

 on starch agar. Nitrates reduced to nitrites, no tyrosinase, no soluble pig- 

 ment. Aerobic, optimum temperature 37° C. 



Actinomyces Bellisari, Dodge, n. nom. 



Streptothrix alba Bellisari, Ann. Ig. Sperim. 14: 467-483, 1904. 



Oospora alba Sartory, Champ. Paras. Homme Anim. 819, 1923. 



Isolated in a warehouse in Naples from the dust of cereal coming from 

 California. Pathogenic to rabbits. 



On agar, colonies amber yellow, very small, not confluent, spores produc- 

 ing a whitish efflorescence which seems limited to the periphery. On potato, 

 growth thick and folded, white, after a week with brownish fissures. No 

 diffusion of color into the medium. On gelatin stab, small white granules ap- 

 pear. These are larger near the surface where the growth expands gradually 

 into a folded white pellicle covering the surface. In broth, colonies small, 

 spherical, isolated, settling and leaving the medium clear. Gelatin slowly 

 liquefied, liquefaction beginning about the twentieth day. 



