k 



ACTINOMYCETEAE 745 



Actinomyces convolutus (Chalmers & Christopherson) Brumpt, Precis 

 Parasitol. ed. 4, 1195, 1927. 



Nocardia convoluta Chalmers & Christopherson, Ann. Trop. Med. Parasitol. 

 10: 223-282, PZs. 8-ii, 1916. 



Discomyces convolutus Neveu-Lemaire, Precis Parasitol. Hum. 44, 1921. 



Oospora convoluta Sartory, Champ. Paras. Homme Anim. 769, 1923. 



Isolated from a yellow grain mycetoma in the Sudan. Not pathogenic for 

 monkeys or other laboratory animals. 



Mycelium less than l/m in diameter. Spores in chains, spherical, 1/i, in di- 

 ameter. No claviform formations. Organism an aero-anaerobe. Gram- 

 positive, not acid-fast. Optimum temperature 30° C, fair growth anywhere 

 between 22° and 37° C. 



Colonies warm buff (Ridgway) before the white efflorescence appears, 

 convoluted, not pigmenting agar media. Growth best on alkaline media. 

 Colony on Sabouraud maltose agar more radiate than convoluted. On potato, 

 good development, convoluted, moist, warm buff with an efflorescence. Colo- 

 nies on gelatin lig'ht buff, small, round ; medium not pigmented. Similar on 

 serum. White efflorescence on milk ; medium not coagulated or digested but 

 is made slightly more alkaline. In peptone broth with glucose, white, non- 

 cohering flocci are deposited, with liquid remaining clear. No acid or gas 

 with glucose, maltose, lactose, sucrose, raffinose, mannitol, or salicin. Serum 

 liquefied. Gelatin not liquefied. 



Actinomyces serratus (Sartory, Meyer & Meyer) Dodge, n. comb. 



Actinomyces asteroides var. serratus Sartory, Meyer & Meyer, Ann. Inst. 

 Pasteur 44: 298-309, 1930. (See also C. R. Acad. Sci. 188: 745-747, 1929.) 



Isolated from a case of actinomycosis of bones with yellow grains. Patho- 

 genic for guinea pigs, but no bone lesions obtained, not even with dog. 



Filaments fragile, branched, segmenting easily, 0.3-0.6/x in diameter. Ar- 

 throspores Ifx in diameter. Gram-positive, spores acid-fast. 



On agar, colonies whitish, verrucose, becoming ochraceous in age, con- 

 fluent into a folded pellicle. On potato, colonies mammillate, whitish, lumpy, 

 becoming powdery, yellowish gray. Serum not liquefied, milk not coagulated 

 but slowly digested. Gelatin not liquefied, no growth in depths. 



Cultural characters suggest A. convolutus Chalmers & Christopherson, but 

 this organism differs in action on serum. 



Actinomyces Donnae Dodge, n. sp. 



Streptothrix sp. Donna, Ann. Ig. Sperim. 14: 449-459, PI. 3, 1904. 



Isolated from sputum in a pulmonary infection. Pathogenic to rabbits. 



Organism aerobic. Colonies small, 2 mm. in diameter, opaque, yellowish 

 white. Growth less on gelatin. In beef broth, there is turbidity with floccose 

 sediment and some evolution of gas. Liquid finally clears. At lower tempera- 

 ture there is no turbidity, and broth remains alkaline. Gelatin not liquefied. 



