k 



ACTINOMYCETEAE 747 



Cultivated from the pus in a fatal infection of the lungs. The sputum was 

 too contaminated with bacteria to permit of easy isolation of the fungus. 

 Pathogenic to guinea pigs. Authors finally decided the infection was a mix- 

 ture of tuberculosis and actinomycosis. 



Terminal portions of the hyphae coiled. Aerobic. Gram-positive. 



Colonies star-shaped, white at first, yellowing and elevated in age, size of 

 lentil, surface rough, center darker, opaque, yellowish, dry. On potato, chalky 

 white granules, confluent, center of colony brownish yellow with chalky white 

 efflorescence, finally brownish. Also grow Avell on Colocasia antiquorum, 

 Neluml)ium setosum, carrot, and Dioscorea japonica, on the first and last sub- 

 strate not adherent. On blood serum, growth not rapid, colonies yellowish and 

 flat. On broth gelatin, growth poor. On broth and Piorkowsky urine-peptone, 

 small gray white or chalk white nodules, often confluent, settling to the bot- 

 tom, no turbidity, never yellowish in color. Surface of milk yellows in 2 days, 

 milk not coagulated or digested. 



Actinomyces pretorianus (Pijper & Pullinger) Nannizzi, Tratt. Micopat. 

 Umana [Pollacci] 4: 38, 1934. 



Nocardia pretoriana Pijper & Pullinger, Jour. Trop. Med. Hyg. 30: 153- 

 156, 2 pis., 1927. 



Isolated from a mycetoma of the shoulder showing yellow grains, 0.5 mm. 

 in diameter, possessing clavate bodies. Pathogenic to guinea pig. 



Hyphae long, O.dfi in diameter, showing marked branching. Organism a 

 strict aerobe, growth best at 37° C, but possible at 22° C. 



Growth circumscribed, in general tending toward orange in pigmentation, 

 no efflorescence, no pigmentation of the medium, adherent. No diastase present. 

 No growth on Sabouraud agar. On potato and simple agar, growth largely 

 buff and elevated, in parts convoluted and orange. Better growth when glyc- 

 erol is added. Growth on carrot convoluted, orange ; on blood agar, buff. On 

 gelatin or blood serum, growth poor, white to orange. In simple broth, glyc- 

 erol broth, or sugar broth, thick, convoluted, bright orange pellicle forms. In 

 hay extract, gray surface films showing small round tufts and a radial struc- 

 ture. Surface of litmus milk is orange, convoluted. No acid or fermentation 

 with any of the usual sugars. Milk coagulated after 10 days. No liquefaction 

 of gelatin or coagulated serum. 



Actinomyces miacrodipodidarum (Fox) Dodge, n. comb. 



Nocardia macrodipodidarum Fox, Disease in Captive Wild Mammals and 

 Birds, 570-595, 1923. 



Found in lumpy jaw with septicemia and gasteroenteritis of kangaroos. 

 Inoculations into experimental animals negative. 



Hyphae up to Ifi in diameter. Gram-positive. 



On blood serum, colonies opaque, pale yellow, circular, discrete, slightly 

 depressed, irregular; no umbilication in center; smooth and slightly glisten- 

 ing for several days, then wrinkled and twisted. Growth on potato is dirty 

 yellow, much wrinkled, friable, adherent. A tough wrinkled scum forms on 



