ACTINOMYCETEAE 749 



Nocardia hicolor Froilauu de Mello & St. Antonio Fernandes, Mem. Asiatic 

 See. Bengal 7: lOG, 1919. 



Found in eerebromeningitis, bronchitis, and lymphadenitis in dog. Patho- 

 genic to white mice, guinea pigs, rabbits, dog, with infection easy. Horse, calf, 

 and birds infected with difficulty, cats not at all. 



On agar, glycerol peptone, or albumose, colonies white at first, then yel- 

 low at the center with Avhite margins. Whole surface wavy and crateriform, 

 crater elevated 3 mm. On potato, growth is in the form of white granules, 

 either discrete or confluent, showing small, grayish white heads on rough 

 spinulose surface; center finally becomes yellow gray; sweet odor developed. 

 On gelatin, colonies white, becoming dirty yellow after several weeks, with 

 the upper layer of the gelatin yellowed. Colonies smaller on coagulated horse 

 serum. In liquid horse serum, small white colonies on the surface, forming a 

 granular pellicle, and at the bottom, woolly, discrete balls. In alkaline broth, 

 the medium remains clear, bottom growth as in serum. Surface growth shows 

 discrete colonies with center yellow and margin white, suggesting a floating 

 composite flower with white ray flowers. Neither coagulated horse serum nor 

 gelatin liquefied. 



It is possible that the following unnamed species belongs here. 



mreptothrix sp. Horst, Zeitschr. Heilk. Abt. Prakt. Anat. 24: 157-176, Ph. 

 13, 14, 1903. 



Isolated from patient with pyemia and brain and bronchial abscesses. Path- 

 ogenic to mice, guinea pigs, and rabbits. 



Helices on potato. No clavate forms in tissues. Organism gram-positive 

 and acid-fast. 



Colonies white, powdery, becoming gray, slightly shining, 2-3 mm. in di- 

 ameter. On glycerol agar, growth is thick, folded, moist; the efflorescence 

 whitish, becoming yellowish, and then reddish with white margin. On serum 

 agar, small granular colonies appear. Colony on potato thick, white, dry, 

 fine-grained, becoming thick, wrinkled, and finally brown. Growth on beet simi- 

 lar. On gelatin, thin, fine, granular, dry colonies. In broth, small white gran- 

 ules from a very thin, fragile pellicle, also small floating colonies in the depths, 

 but no turbidity. Growth much less in sugar broth, and poor in peptone 

 solution. Organism grows on milk as a thick sulphur yellow, later orange, 

 pellicle, without altering the medium. Gelatin not liquefied. 



Actinomyces brasiliensis (Lindenberg) Gomes, Ann. Palistas Med. Cirurg. 

 14: 150-156, 1923. 



Discomyces hrasiliensis Lindenberg, Rev. Med. Sao Paulo Sept. 30, 1909 ; 

 Arch, de Parasitol. 13: 265-282, 3 figs., 1909. 



Nocardia hrasiliensis, Castellani & Chalmers, Man. Trop. Med. ed. 2, 816, 

 1913. 



Streptofhrix hrasiliensis Greco. Origine des Tumeurs . . . 724, 1916. 



Oospora hrasiliensis Sartory, Champ. Paras. Homme Anim. 786, 1923. 



