MISCELLANEOUS FUNGI IMPERFECTI 849 



Colonies on Sabouraud agar black, spherical, ovoid or irregular cells of 

 deep greenish color, without mycelium. In subcultures, colonies are circular 

 with raised center, black with a mouse gray velvet of young mycelium. Fasci- 

 cles of hyphae appear. As culture becomes older, color becomes grayer, sur- 

 face velvety and grooved. Growth good on bread or sugar agar, poor on rice, 

 also on serum, where hyphae are not formed. Growth best at 37° C, very 

 slow at room temperature. Growth good on the following medium: Peptone 

 2 gm., agar 2 gm., honey 8 gm., water 100 c.c. No gas on sugars. Slight 

 acidity with sucrose, mannite, dulcite, galactose, maltose, glucose, or starch. 

 Milk not coagulated, gelatin liquefied. 



Doubtful Species 



Oidium coeruleus cuticularis Greco, Argentina Med. 5 pis., 1909; Origine 

 des Tumeurs 54-63, Figs. 11-14, 1916. 



Found on a young shepherd who was engaged in dipping sheep to cure 

 them of the scab. Lesion began as red spots of desquamation, preceded by 

 small vesicles arranged in the arc of a circle, gradually spreading to the fore- 

 arms, pruriginous. Surface erythematous, red or somewhat lilac colored, 

 slightly desquamatous, formed by the confluence of plaques still smaller, 

 united more or less tangentially by their edges which are shown by the curved 

 lines of small gray crusts, very adherent to a red surface, slightly redder than 

 the central portions of the plaques covered with small furfuraceous grayish 

 white scales. 



Cells spherical or ovoid, about 6/x in diameter, forming filaments 8-10 x 4/*, 

 in 24 hours, which elongate to 120)U long by 1/a thick. Cells 5-6/^ long. Fila- 

 ments variously branching, spores terminal on branches, with whole filament 

 later breaking up into arthrospores. 



On Sabouraud glucose, growth is visible in 24 hours, colonies at 8 days 

 1 cm. in diameter, elevations 2-3 mm., color olive green, umbilicate with ele- 

 vated margin, finally becoming dark green, almost black ; dry and separating 

 from the medium with difficulty. Growth on Sabouraud maltose much the 

 same as on Sabouraud glucose. Colonies on potato glycerol or plain potato 

 abundantly cover the surface with a grayish, greenish olive color, powdered with 

 black spores ; liquid filled Avith flocculent floating colonies, with grayish olive 

 centers and white margins; liquid finally covered with dark olive green pel- 

 licle, with a dirty gray powdery surface. Growth in broth or glycerol broth 

 much the same as in the liquid of potato glycerol. 



This organism is problematical. The lesion suggests Epidermophyton 

 while the description of colony, with black spores, suggests Hormodendron 

 or some organism of that group. 



Blastomyces sp. Rudolf, Arch. Schifi:'s.-Tropenhyg. 18: 498, 1914. 



Found in the disease known as "Figueim" in Minas Geraes and Goyaz 

 in Brazil (mossy foot?). Isolated from warts on back of foot causing a 

 cauliflower papilloma. Pathogenic to monkeys and white rats. 



Colonies on Sabouraud agar dark brown to black, of appearance of 

 mouse skin. 



