ACTINOMYCETEAE 735 



potato, colony dry, membranous, folded, dirty white, growing along walls of 

 tube, finally reticulate, brownish. On carrot, colony similar, folds thinner 

 and disappearing in age ; yellowish white becoming brownish yellow. On 

 broth, pellicle white, folded, adherent, medium remaining clear. On sugar 

 broths similar, but folds more highly developed. On vegetable broth, pellicle 

 grayish white, sediment sandy. Milk coagulated and digested, with yellowish 

 white pellicle. Litmus milk decolorized. No diastatic action. 



Actinomyces Tarozzii (Miescher) Dodge, n. comb. 



Streptothrix Tarozzii, Miescher, Arch. Derm. Syphilis 124; 297-442, Pis. 

 20-25, 1917. 



Actinomyces alhus Tarozzi, Archivio Sci. Med. 33: 553-632, 1909. 



Isolated from a case of Madura foot with yellowish white granules. Path- 

 ogenic to laboratory animals. 



Hyphae 0.5-1.5//. in diameter, repeatedly dichotomously branched, occa- 

 sionally septate. Chlamydospores terminal, spherical, single, 4-5/x in diam- 

 eter. This organism is a strict aerobe, grows at 15°-20°, better at 37° C. 

 Gram-positive, not acid-fast. 



GroAvth on agar opaque, glassy, smooth, adherent with, sometimes, a white 

 efflorescence. Colonies on glycerol agar velvety, snow white, soon confluent. 

 On potato or potato glycerol, colonies hemispheric, concave below, adherent to 

 the substrate, finally, confluent into a mammillate white, velvety colony, turning 

 brown and black below, remaining white above. Dark color not formed on 

 new potatoes. Velvety white colonies on gelatin. On coagulated blood serum, 

 development is slow, with the small hemispheric colonies adherent to the sub- 

 strate and becoming dry without the formation of aerial hyphae. In broth 

 cultures, both beef and potato, the deposit is composed of small gelatinous 

 colonies which are finally confluent into a mucoid mass. Surface colonies de- 

 velop a white aerial mycelium with liquid remaining clear, moldy odor. No 

 growth on hay infusion. Gelatin slowly liquefied. 



Actinomyces Ribeyro, Dodge, n. sp. 



Hongo artrosporado Ribeyro, Ann. Fac. Med. Lima 3: 1-5, 1 pi., 1919. 



Oospora sp. Sartoiy, Champ. Paras. Homme Anim. 810, 1923. 



Isolated from a generalized infection on the arms, legs, and chest in a 

 patient from Cotabambas, Apurimac, Peru. There were from 30 to 40 dis- 

 crete pustules in various stages of development. These start as small red 

 papules, become acuminate, 1-2 cm. in diameter, yellowing with the central 

 eminence and serous content darkening. On rupture, a dark crust forms and 

 a lively burning pain is provoked for a few days. Finally the crust disap- 

 pears, without leaving a sear. Not pathogenic to rabbit except on subcutane- 

 ous inoculation, when typical nodules are produced. 



In tissues, coccobacilli, 1-2 x 1/x, straight or slightly curved. Mycelium 

 fine, branched, 0.5/a in diameter, not septate. Sporulation in about 20 days. 



Colonies slightly elevated, creamy in consistency and color, with a white, 

 powdery efflorescence. Very little or no growth on Sabouraud agar. In broth, 



