750 MEDICAL MYCOLOGY 



Isolated from a mycetoma of the thigh and leg (10 cm. below inguinal 

 fold to upper portion of the foot with movement of the knee inhibited), Brazil. 

 Not inoculable to ordinary laboratory animals. 



Hyphae O.dfi in diameter, spores 1-2/x, in diameter, spherical to ovoid. Op- 

 timum growth at room temperature [tropical]. Aerobic. 



Colonies on ordinary agar opaque, chalky white to yellow, being white at 

 the center and orange ochraceous at the margin, showing concentric zoning. 

 Colonies on Sabouraud agar at 37° C, rose violet. On Sabouraud glucose, 

 growth poorer than on ordinary agar, white at room temperature, rose violet 

 at 37° C. On potato as on agar, orange yellow with radiating furrows; me- 

 dium colored brown. Colony on serum, whitish at 37°, yellowish at room 

 temperature. Good growth on egg. Milk shows an orange yellow growth, 

 pellicle on surface, no coagulation. Slight growth on broth at 37°, hay in- 

 fusion shows filamentous flocci. Gelatin not liquefied. 



Chalmers & Christopherson relegate this species to Actinomyces asteroides, 

 but it differs in the nature of the grain (soft in A. hrasiliensis) and the colony 

 on Sabouraud agar here is wholly rose -vdolet, while that of A. asteroides is 

 yellow at the center and red at the periphery on this medium. 



Castellani & Chalmers, Man. Trop. Med. ed. 3, 1058, 1919, also give this as 

 synonym of Nocardia asteroides, as do also Neveu-Lemaire, and Pollacei & Nan- 

 nizzi. 



Actinomyces asteroides (Eppinger) Gasperini, Centralbl. Bakt. I, 15: 84, 

 1894. 



Cladothrix asteroides Eppinger, Wiener Klin. Woch. 3: 321-323, 1890; 

 Beitr. Path. Anat. [Ziegler] 9: 287-328, Pis. 13, 14, 1891. 



Streptothrix asteroides Gasperini, 1891; Petri. N. Giom. Bot. Ital. n. s. 10: 

 591, 1903. 



Streptothrix Eppi^igeri Rossi-Doria, Ann. 1st. Ig. Sperim. Univ. Roma. 

 N. S. 1: 399-438, PI. 8, 1891. 



Oospora asteroides Sauvageau & Radais, Ann. Inst. Pasteur 6: 250, 1892. 



Nocardia asteroides Blanchard, in Bouchard, Traite Path. Gen. 2: 811-926, 

 1895. 



Diseomyces asteroides Gedoelst, Champ. Paras. Homme Anim. 173, 174, 

 1902. 



Streptothrix Freeri Musgrave & Clegg, Philippine Jour. Sci. B. 2: 447-511, 

 1907, was referred here by Chalmers & Christopherson, Ann. Trop. Med. Para- 

 sitol. 10: 255, 1916. 



This organism is an aerobe (Poulerton-Jones), aero-anaerobe (Chalmers 

 & Christopherson), or anaerobe (Castellani) found in mycetoma (Castellani) 

 in brain abscess from a case of meningitis, and in diffuse peritonitis (Mac- 

 Callum). The original case was pseudotuberculosis with cerebrospinal menin- 

 gitis. Pathogenic to rabbits, guinea pigs, and monkeys with pseudotuberculo- 

 sis following intraperitoneal inoculation. Cultures soon lose their virulence. 

 Rossi-Doria found them nonpathogenic. 



