ACTINOMYCETEAE 751 



Mycelium 0.2/x. in diameter, spores nearly spherical and in chains. 



On agar, colonies verrucose, becoming confluent and erateriform or ir- 

 regularly folded, pale yellow becoming ochraceous in age, 3-4 mm. in diameter, 

 adherent. Colonies similar on glucose or glycerol agar, but growth is more 

 rapid. On potato, colonies are elevated and verrncose, white at first, then 

 brick red, color darker at the center, then covered with a white conidial efflo- 

 rescence. On gelatin and serum, culture yellow, raised and wrinkled, no pig- 

 mentation of the medium. In peptone beef broth, liquid remains clear with a 

 sediment of small, very thin scales at the bottom of the tube, and a thin waxy, 

 fragile pellicle. Litmus milk is at first acidified, then becomes alkaline with 

 the formation of a yellowish granular sediment. Litmus whey decolorized, 

 with the formation of a thick pellicle and sediment. In a mixture of agar and 

 hydrocele fluid, growth is thick, homogeneous, with a yellowish white film 

 over the whole surface. Milk not coagulated. Gelatin and coagulated serum 

 not liquefied. 



Actinomyces Sanfelicei (Redaelli) Nannizzi, Tratt. Micopat. Umana [Pol- 

 lacci] 4: 51, 1934. 



Nocardia Sanfelicei Redaelli, 1st. Sieroterapico Milanese 7: 75-101, 121-135, 

 Pis. 1-8, 1928. 



StreptothtHx acido-resistente Sanfelice, Boll. 1st. Sieroterapico Milanese 2: 

 327-331, 1922. 



Isolated from fatal lesions in rat ; source unknown. Pathogenic to guinea 

 pig, rabbit, rat, etc. 



Growth at temperatures between 15° and 40° C, optimum 37° C. Aerobic, 

 gram-positive, usually acid-fast. 



Growth on solid media best in those containing glycerol, colony semitrans- 

 parent, margins irregular, surface minutely verrucose, becoming thick rugose, 

 yellowish, not adherent. On carrot agar, colonies round, elevated, folded to 

 cerebriform. On Petragni & Petroff medium, development is more abundant, 

 with thick, yellowish, finely mammillate colonies, margins thin, smooth, semi- 

 transparent, festooned. On potato as on agar. Colonies on potato glycerol 

 yellowish red, thick, irregularly rugose, very adherent. On liquid media, thin 

 grayish pellicle becoming thick, yellowish, and rugose; small, hard, granular 

 colonies in sediment. No fermentation of sugars, no liquefaction of gelatin, 

 no coagulation of milk. Slight acidity on sugar broths (except lactose). 



Actinomyces spumalis (Sartory) Dodge, n. comb. 



Oospora spumalis Sartory in Sartory & Bailly, Mycoses pulmonaires 318- 

 321, 1923. 



Isolated from sputum. Patient recovered under iodide treatment. Path- 

 ogenic to guinea pig. 



Growth at 37°. Mycelium 0.4-0.5// in diameter, irregularly branched. 

 Terminal ehlamydospores, often in chains, 0.8//, in diameter. Helices of 4-5 

 turns abundant in older cultures. 



