ACTINOMYCETEAE 753 



Growth under aerobic conditions as follows: On agar, growth slow. 

 Large colonies, white, opaque, circular, with the center elevated in the shape 

 of a hemisphere and the marginal zone flat. The small colonies are more ir- 

 regular, not adherent but penetrating the substrate, some becoming crateri- 

 form; aerobic subcultures rapidly dying. Growth similar on coagulated serum. 

 Usually no growth on potato, but occasionally small, grayish white colonies of 

 gram-positive filaments. No growth on potato-glycerol. In broth, there ap- 

 pear grayish white, fine grains, slightly adherent to the walls of the tube, and 

 an abundant floceose deposit ; the medium remains clear. In serum broth, 

 growth better but otherwise the same. No growth on gelatin at 22° C. At 

 37° C, groAvth as in broth. In hay infusion, growth as in broth. Milk is 

 coagulated slowly, with the whey becoming acid. Curd not digested. Neither 

 coagulated serum nor gelatin liquefied. No indol formation. 



Anaerobic cultures show some variations. On agar, colonies round, ele- 

 vated, shining, moist, whitish, 2-3 mm. in diameter, not adlierent, no efflores- 

 cence, odor of hydrogen sulphide. No growth on potato. Growth on gelatin 

 and broth as in aerobic, except that with the latter there is also produced the 

 odor of hydrogen sulphide. 



Actinomyces cruoris (Macfie & Ingram) Brumpt, Precis Parasitol. ed. 4, 

 1195, 1927. 



Nocardia cruoris Macfie & Ingram, Ann. Trop. Med. Parasitol. 15: 283-285, 

 1921. 



Oospora cruoris Sartory, Champ. Paras. Homme Anim. 809, 1923. 



Isolated from the heart blood of a patient, both of whose lungs were af- 

 fected with a bronchopneumonia and whose case had otherwise presented the 

 clinical picture of encephalitis lethargica. Necropsy findings given in detail. 

 Pathogenicity not proved. Guinea pigs not affected after 7 months. 



Hyphae freely branching, nonseptate, Ifx or less in diameter, in old cul- 

 tures fragmenting with ends of branches clavate. 



No growth on glucose or maltose agar. Growth on blood agar or ''nas 

 agar" at 26° or 37° C, slowly spreading, smooth and dome-shaped, later opaque 

 and puckered in the middle, radially striate and semitransparent at the periph- 

 ery. In early cultures, the efflorescence is abundant and blue. In later sub- 

 cultures, it is scanty and gray or white, medium not pigmented. Gro\vth slow 

 and feeble under anaerobic conditions. Cultures have no distinctive odor. On 

 potato, growth is abundant, whitish, developing a brown or gray brown efflores- 

 cence and staining medium dark brown. Growth also good on coagulated blood 

 serum with substrate also being pigmented dark broAvn. No growth on gel- 

 atin, broth, or peptone water. No change in arabinose, rhamnose (isodulcite), 

 galactose, glucose, fructose, mannose, lactose, maltose, sucrose, raffinose. 

 starch, dextrin, glycogen, inulin, amygdalin, helicin, phlorhizin, salicin, glyc- 

 erol, erythritol, adonitol. dulcitol, inosite, mannitol. sorbitol. Litmus milk un- 

 changed. Coagulated serum not liquefied. 



