230 PROCEEDINGS OF THE NATIONAL MUSEUM vol. lu 



UCD: University of California at Davis, Calif. (A. T. McClay). 



UCLA: University of California at Los Angeles, Calif. (W. A. McDonald). 



UCR: University of California at Riverside, Calif. (P. H. Timberlake). 



UI: University of Idaho, Moscow, Idaho (W. F. Barr). 



UK: University of Kansas, Lawrence, Kans. (R. H. Beamer). 



U. Mass: University of Massachusetts, Amherst, Mass. (C. P. Alexander). 



UM: University of Minnesota, Minneapolis, Minn. (E. F. Cook). 



U. Miss: University of Mississippi, University, Miss. (F. M. Hull). 



UN: University of Nebraska, Lincohi, Nebr. (L. W. Quate). 



USAC: Utah State Agricultural College, Logan, Utah (G. F. Knowlton). 



USNM: U.S. National Museum, Washington, D.C. (C. W. Sabrosky). 



UT: University of Tennessee, Knoxville, Tenn. (H. Howden). 



UU: University of Utah, Salt Lake City, Utah (G. F. Edmunds, Jr.). 



UW: University of Wisconsin, Madison, Wis. (C. L. Fluke). 



VNM: Naturhistorisches Museum, Vienna, Austria (M. Beier). 



WCB: W. C. Bentinck collection, Berkeley, Calif. (W. C. Bentinck). 



WSC: Washington State College, Pullman, Wash. (M. T. James). 



ZI: Zoologisches Institut, Berlin, Germany (F. Peus). 



ZSI: Zoological Survey of India collection, Calcutta, India (A. P. Kapur). 



Methods 



It was found desirable to prepare permanent slide mounts of the 

 male genitalia, since other preservative methods were nearly as much 

 work and were much less permanent. Such methods as placing the 

 genitalia on card points or attaching them to the pin in small vials 

 containing glycerine were discarded in favor of slide mounts when it 

 was discovered that the solvent "Cellosolve" would rapidly dissolve 

 many-year-old balsam mounts, even though they had been pre- 

 hardened in a hot oven. 



To dissect out the male genitalia the specimen is relaxed (after 

 first removing all printed labels) in a petri dish containing distilled 

 water and carbolic acid (to prevent molds). Rapid relaxing can be 

 obtained by placing the petri dish under a light for 30 minutes or 

 more. The specimen is then removed, turned venter up, and the 

 head of the pin is inserted into a pinning block to a point where the 

 dorsum of the specimen just touches the block. Using delicate 

 forceps in one hand to hold the lateral tip of the abdomen, and insert- 

 ing a small, sharply curved, minuten pin into the intersegmental 

 membranes around the genitalia with the other hand, the genitalia 

 can be eased out Avithout extra injury to the specimen. The genitalia 

 are then placed in distilled water, examined to see if all the parts 

 were removed, and then placed in a 10 percent solution of potassium 

 hydroxide (KOH). After warming for 5 to 15 minutes in this solu- 

 tion, the genitalia are removed and again placed in distilled water. 

 At this time the parts are dissected and transferred into 70 percent 



