Nutrition Investigations. 307 



I. The Pentosans : 



Dulse {Rhydomenia palmata), 



Limu Lipoa {Haliseris pardalis), 



Limu Eleele {Enter omorpha intestinalis) , 



Limu Pahapaha {Ulva laduca laciniata and Uivafasciata). 



II. The Galactans: 



Irish Moss {Chondrus crispus), 



Limu Manauea {Gracilaria cor onopij olio), 



Limu Huna {Hypnea nidifica), 



Limu Akiaki {Ahnfeldtia concinna), 



Limu Uaualoli {Gymnogongrus vermicularis americana and 



Gymnogongrus disciplinalis) , 

 Limu Kohu {As parages pis sanfordiana), 

 Slippery Elm {Ulmus fuha). 



III. The Mannans: 



Salep (Species of Orchis and Eulophia). 



IV. The Levulans: 



Sinistrin {Urginea or Scilla maritima). 



The primary object of these investigations has been to determine 

 the fate of these substances in the alimentary canal of man, since they 

 are all used as foodstuffs except sinistrin, and are all representative 

 of a large class of materials so employed. The experiments con- 

 ducted have been Chemical, Bacteriological and Physiological 

 in character, and each of these phases will be taken up separately in 

 turn in the following pages. 



Chemical Investigations. 



The aim of the experiments was to isolate, identify, and pre- 

 pare for bacteriological and physiological experiments, any water- 

 soluble carbohydrates present in sufficient amount in the materials 

 under consideration; and to determine such of their properties as 

 would facilitate their detection, isolation, and quantitative estimation 

 in these experiments. 



general methods. 



All the seaweeds, with the exception of Irish moss, were washed re- 

 peatedly in cold tap water, to remove salt, sand, and other foreign 

 substances, and for convenience, dried by spreading in thin layers 



