Nutrition Investigations. 



331 



in strength with the loss of unaltered carbohydrate, as shown in the 

 following summary: 



Salep. 



CULTURES USED. 



REDUCTION OF 

 FEHLING 'S 

 SOLUTION. 



aOTATION OF UNALTERED CARBO- 

 HYDRATE AFTER HYDROLYSIS. 



Salep. 



Control. 



Mixture of Pure Aerobes. 



Mixture of Faecal and Soil Bacteria 

 (aerobic) 



Mixture of the Bacilli of Malignant 

 Oedema and Symptomatic Anthrax . . 



IMixture of Faecal and Soil Bacteria 

 (anaerobic) 



(Insoluble 

 jelly) 



+ 



+ 



+ 



Not det 

 + 0.17° 

 + 0.13° 

 + 0.03° 



ermined 



+ 0.20° 



+ 0.20° 



+ 0.20° 



These experiments give some grounds for expecting the hydrolysis 

 of salep in the alimentary tract, through the action of bacteria. 

 Two experiments with sinistrin gave the following results: 



Sinistrin. 



Sinistrin is therefore hydrolyzed by the anaerobic putrefactive 

 organisms, but further experiments are necessary to determine how 

 readily this change takes place. 



Physiological Investigations. 



In the physiological experiments, attempts have been made to 

 answer the following questions: (1) To what extent are hemicelluloses 

 digested by animal and vegetable enzymes? (2) Can they be ab- 

 sorbed and utilized without intervention of the alimentary tract? 



