Nutrition Investigations. 



337 



for the presence of carbohydrate was made by testing the urine for 

 reducing substances, before and after hydrolysis. The results are 

 shown in the following table: 



Examination of Urine. 



REDUCTION OF FEHLING 's 

 SOLUTION. 



Before 

 Hydrolysis. 



After Hydroly- 



October 13, 11 A.M 



October 13, 12:30 P. M I Injection 



October 13, 2 P.M j 27 



October 13, 5 P.M (30 



October 14, 9 A.M 450 



October 15, 5 P.M 45 



October 16, 9:30 A.M I — 



The urine before the injection showed a rotation of —0.14°, a 

 sample of the mixed urines of October 13, 5 P.M., and October 14, 

 9 A.M., showed a rotation of —0.034°, the diminished levo-rota- 

 tion undoubtedly due to the presence of this dextro-rotatory carbohy- 

 drate. On hydrolysis, 50 cc. of this mixed sample yielded sugar 

 equivalent to 0.035 grams of dextrose (by Allihn's method). From the 

 remainder of this sample, Irish moss carbohydrate was isolated; it 

 formed a grayish-white powder, swelling in water, and yielding mucic 

 acid on oxidation with nitric acid. 



Experiment B. - A dog weighing 9 kg. received intraperitoneally 

 100 cc. of a 2 per cent solution of Irish moss preparation. Examina- 

 tion for carbohydrate was made as in the preceding experiments. 

 The results appear in the following table: 



Examination of Urine. 



Irish moss was isolated and identified in the urine of November 1st. 



