338 



Mary Davies Swartz, 



INJECTIONS OF SALEP. 



I. Subcutaneous. 



A dog weighing 7.2 kg. received 56 cc. of salep solution, containing 

 0.75 grams of pure mannan. No reducing substance was found in 

 the urine. The changes in rotation, due to salep, are shown in the 

 following table : 



Examination of Urine. 



ROTATION. 



ESTIMATION OF AMOUNT 

 OF SALEP EXCRETED. 



May 17, 



May 18, 3:30 P.M 

 May 19, 9 A.M... 

 May 20, 9 A.M... 

 May 21, 9 A.M... 

 May 22, 9 A.M... 

 May 22, 5P.M... 



Injection 



138 

 132 

 114 

 127 



0.17' 



- 0.27° 

 -0.27° 

 -0.20° 

 -0.14° 



Grams. 



0.3 

 0.3 

 0.04 



Salep was isolated and identified in the urines of May 20, 21, and 22. 



2. Intraperitoneal. 



Experiment A. A dog weighing 7 kg. received 68 cc. of salep solu- 

 tion, containing 1.2 grams of air dry mannan. No reducing substance 

 was present in the urine at any time. Tests for the presence of salep 

 by means of Fehling's solution, gave the following results: 



Examination of Urine. 



The salep was easily isolated and identified in the urine of October 

 22 and 23, the sugar obtained on hydrolysis being equivalent to 0.33 

 grams salep. 



Experiment B. A dog weighing 9.2 kg. received 80 cc. of salep so- 



