Nutriiion Investigations. 



339 



lution, containing 1.4 grams of air dry substance. No reducing sub- 

 stance was detected in any of the urines. Tests for salep with Feh- 

 ling's solution gave the following results: 



Examination of Urine. 



SALEP PRESENT. 



October 24, 11 A.M 

 October 24, 12 M.. 

 October 25, 12 M . . 

 October 26, 10 A.M 

 October 27, 10 A.M 

 October 28, 10 A.M 



Injection 

 155 

 180 

 180 



+ 

 + 

 + 



From the urine of October 25, salep was isolated, which yielded on 

 hydrolysis 0.39 grams reducing sugar as dextrose; it was also isolated 

 from the urines of the next two days, but was not estimated quanti- 

 tatively. 



Experiment C. A dog weighing 9.2 kg. received 90 cc. of salep 

 solution, containing 1.8 grams of pure mannan. No reduction of 

 Fehling 's solution occurred with any of the samples. Tests for salep 

 with Fehling's solution gave the following results: 



Examination of Urine. 



VOLUME. ! ROTATION. 



SALEP PRESENT. 



December 2, 10 A.M . . 

 December 2, 2:30 P.M 

 Decembers, 10 A.M.. 

 December 4, 10 A.M . . 

 December 5, 10 A.M . . 



Injection 

 960 

 234 

 520 



-0.17' 



-0.41° 

 -0.27° 



+ 

 + (0.6gm.) 

 + (0.5gm.) 



Unfortunately this experiment was unavoidably interrupted at 

 this point. The salep was precipitated from 50 cc. of the urine for 

 December 3, hydrolyzed, and sugar determined gravimetrically as 

 dextrose, from which the total amount of salep in this day's urine was 

 calculated as 0.67 gram. Salep determined in the same way on De- 

 cember 4, showed an elimination of 0.18 gram; hence 0.85 gram was 

 actually recovered in these two days. The influence of the \e\o- 

 rotatory carbohydrate on the rotation of the urine was marked. 



