340 



Mary Davies Swartz, 



Experiment D. A dog weighing 6.4 kg. received 98 cc. of salep 

 solution containing 1 gram of pure mannan. No reduction of Feh- 

 ling's solution was observed throughout the experiment. The changes 

 in rotation due to the salep are shown in the following table: 



Examination of Urine. 



salep precipitated by 

 fehling's solution. 



January 31 

 February 1 

 February 2 

 February 3 

 February 4 

 February 5 



116 

 Injection 

 152 

 238 

 154 

 137 



- 0.41° 



-0.41° 

 -0.13° 

 -0.13° 



- 0.20° 



+ 



The results in this experiment are very puzzling. The normal rota- 

 tion was high ( — 0.41°) for several weeks before this experiment 

 but fairly constant, averaging —0.44°. If salep were excreted as 

 mannan, the levo-rotation should have increased, yet it was decid- 

 edly low on a day when salep was shown to be present, and also on a 

 day when none could be detected. The absence of any positive tests 

 for sugar, excluded the idea that the salep was being excreted in this 

 form, but finally a sample of February 4, was tested with yeast, and 

 marked fermentation observed. Unfortunately, this was after all the 

 other samples had been discarded, hence no further tests could be 

 made. 



Experiment E. A dog weighting 9.8 kg. received intraperitoneally 

 97.5 cc. of salep solution containing 1.3 grams pure mannan. No re- 

 duction of Fehling's solution was observed. The changes in rotation 

 are shown in the first table on the next page. 



Salep was isolated and identified as carbohydrate, in the urines of 

 May 19, 20, and 21, although the amount in the last two days was ap- 

 parently too small to be detected by any change in the rotation. 



INJECTIONS OF SINISTRIN. 



/. Subcutaneous. 



A dog weighing 6.5 kg. received 49 cc. of sinistrin solution, contain- 

 ing 3.3 grams pure substance. This solution showed a rotation of 



